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Interleukin 16 (IL16) antibody

Antigen

Interleukin 16 (IL16)
Synonyms LCF, IL-16, prIL-16, FLJ16806, FLJ42735, FLJ44234, HsT19289, KIAA4048, mKIAA4048, IL16
Clonality Monoclonal (G11-II)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
2 references available
Catalog no. ABIN111991
Quantity 0.2 mg  (1.0 mg/ml)  (Variants)
Price 715.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Interleukin-16 / IL16
UniProt Q14005
Immunogen Recombinant Human IL-16
Cross-Reactivity Human
Isotype IgG1
Clone G11-II
Description The leukocyte chemotactic factor (LCF) or interleukin-16 is a proinflammatory cytokine andnatural soluble ligand to the human CD4 molecule. LCF is produced by CD4+ and CD8+ Tlymphocytes and is considered essential to the influx of CD4+ T lymphocytes andmacrophages into an inflammatory lesion. LCF has been detected in lymphocytes andmicroglial cells of multiple sclerosis lesions A large neuronal precursor (NIL-16) hasrecently been identified which can be detected in the cytosol of neurons in the cerebellumand hippocampus.
Characteristics Synonyms: IL-16, LCF, Lymphocyte chemoattractant factor
Specificity G11/II detects LCF (IL-16), a homotetrameric glycoprotein of 56 kDa molecular weight that iscomposed of four noncovalently linked peptides of 14 kDa. Species: Human. Others not tested.

Application Details
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Protocol Protocol with Formalin-Fixed, Paraffin-Embedded Sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Place slide in a cuvette with 250ml 0. 01M citrate buffer, pH 6. 03. Heat slide in a microwave oven for 2 x 7 min. at 700 Watt4. Leave slide in the buffer for 20min for cooling5. Wash in distilled water6. Block endogenous peroxidase7. Wash in PBS8. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber10. Wash in PBS11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 14. Wash in PBS 15. Counterstain with Mayer's hemalum
Application Notes Immunohistochemistry on Paraffin Sections: 2.5-5 µg/ml (1/200-1/400). Microwavepretreatment in citrate buffer is recommended for antigen retrieval. Recommneded Positive Control: Human spleen. Has been described to work in ELISA and Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Purification Purified
Buffer PBS, pH 7.2 containing 0.09% Aodium Azide as a preservative and nostabilizer.
Storage Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Restrictions For Research Use only

Publications
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Schluesener, Seid, Kretzschmar et al.: "Leukocyte chemotactic factor, a natural ligand to CD4, is expressed by lymphocytes and microglial cells of the MS plaque." in: Journal of neuroscience research, Vol. 44, Issue 6, pp. 606-11, 1996 (PubMed).

Kurschner, Yuzaki: "Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 18, pp. 7770-80, 1999 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Interleukin 16 (IL16)", type "Antibodies"
Hosts Rabbit (62), Mouse (41), Chicken (2)
Reactivities Human (88), Mouse (Murine) (42), Rat (Rattus) (41), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)
Applications ELISA (65), Western Blotting (WB) (54), Immunofluorescence (IF) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (IHC) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Functional Studies (Func) (5), Immunoprecipitation (IP) (3), Neutralization (Neut) (3), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), Blocking Antibody (Inhibition) (1)
Conjugates Biotin (5), HRP (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
Epitopes N-Term (7), AA 502-631 (2), C-Term (2), AA 503-631 (1)