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Interleukin 16 (IL16) antibody

Details for Product No. ABIN111991
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Antigen
Synonyms IL16, LCF, NIL16, PRIL16, prIL-16, mKIAA4048, IL-16
Reactivity
Human
(137), (46), (43)
Host
Mouse
(84), (63), (3), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(99), (98), (26), (20), (14), (7), (5), (4), (3), (3), (3), (2)
Pubmed 3 references available
Quantity 0.2 mg
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Catalog No. ABIN111991
621.50 $
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Immunogen Recombinant Human IL-16
Clone G11-II
Isotype IgG1
Specificity G11/II detects LCF (IL-16), a homotetrameric glycoprotein of 56 kDa molecular weight that is composed of four noncovalently linked peptides of 14 kDa.
Purification Affinity Chromatography
Alternative Name Interleukin-16 / IL16
Background The leukocyte chemotactic factor (LCF) or interleukin-16 is a proinflammatory cytokine and natural soluble ligand to the human CD4 molecule. LCF is produced by CD4+ and CD8+ T lymphocytes and is considered essential to the influx of CD4+ T lymphocytes and macrophages into an inflammatory lesion. LCF has been detected in lymphocytes and microglial cells of multiple sclerosis lesions A large neuronal precursor (NIL-16) has recently been identified which can be detected in the cytosol of neurons in the cerebellum and hippocampus.
Alternate names: IL-16, LCF, Lymphocyte chemoattractant factor
Gene ID 3603
NCBI Accession NP_001165599.1
UniProt Q14005
Application Notes Immunohistochemistry on Paraffin Sections: 2.5-5 µg/ml (1/200-1/400). Microwavepretreatment in citrate buffer is recommended for antigen retrieval. Recommneded Positive Control: Human spleen. Has been described to work in ELISA and Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Protocol with Formalin-Fixed, Paraffin-Embedded Sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Place slide in a cuvette with 250ml 0. 01M citrate buffer, pH 6. 03. Heat slide in a microwave oven for 2 x 7 min. at 700 Watt4. Leave slide in the buffer for 20min for cooling5. Wash in distilled water6. Block endogenous peroxidase7. Wash in PBS8. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber10. Wash in PBS11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 14. Wash in PBS 15. Counterstain with Mayer's hemalum
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 0.09% Aodium Azide as a preservative and no stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time, it does not show decline in activity after two weeks at 4°C.
Expiry Date 12 months
Background publications Schluesener, Seid, Kretzschmar et al.: "Leukocyte chemotactic factor, a natural ligand to CD4, is expressed by lymphocytes and microglial cells of the MS plaque." in: Journal of neuroscience research, Vol. 44, Issue 6, pp. 606-11, 1996 (PubMed).

Kurschner, Yuzaki: "Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 18, pp. 7770-80, 1999 (PubMed).

Corcoran, Monat, Pelusi et al.: "Optical signal processing on a silicon chip at 640Gb/s using slow-light." in: Optics express, Vol. 18, Issue 8, pp. 7770-81, 2010 (PubMed).

Validation Images
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