BUB1A (BUB1A) antibody
| Antigen |
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| Synonyms |
C86192, AI323730, AL022789, Ab2-098, zgc:92230, MGC127743 |
| Clonality |
Monoclonal (14H5) |
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Host
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Reactivity
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Application
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 Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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| Catalog no. |
ABIN112048 |
| Quantity |
0.1 ml (1.1 mg/ml) (Variants) |
| Price |
490.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1
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Alternative name
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BUB1A
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Swiss-Prot
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O43683
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Immunogen
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Recombinant human BUB1/CFA.
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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14H5
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Description
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BUB1 is a kinase involved in spindle checkpoint function. The kinase functions in part byphosphorylating a member of the miotic checkpoint complex and activating the spindlecheckpoint. Mutations in BUB1 have been associated with aneuploidy and several forms ofcancer.
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Specificity
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Human BUB1. Not tested for reactivity with rodents or other vertebrates.
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Protocol
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Immunofluorescence protocol - Formaldehyde fixationCollect cells from T. c. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperatureon an orbital shaker.
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Application Notes
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Suitable for Immunoprecipitation, Immunofluorescence and Western blot. Any human cellline can be used as a positive control. Subcellular localization of antigens are onlydetected in mitotic cells. Dilution: try 1 µg/ml final concentration. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.1 mg/ml
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Buffer
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PBS with 0.09% sodium azide as preservative.
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Storage
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Store the antibody at 2-8°C for one month or (in small aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Research Area
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Cell Cycle
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Restrictions
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For Research Use only
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Publications
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Zhou, Gupta, Yao et al.: "Paclitaxel-resistant human ovarian cancer cells undergo c-Jun NH2-terminal kinase-mediated apoptosis in response to noscapine." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39777-85, 2002 (PubMed).
Liu, Hittle, Jablonski et al.: "Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis." in: Nature cell biology, Vol. 5, Issue 4, pp. 341-5, 2003 (PubMed).
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Alternatives for antigen "BUB1A (BUB1A)", type "Antibodies"
