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Details for Product No. ABIN112054

Gem (Nuclear Organelle) Associated Protein 2 (GEMIN2) antibody

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Synonyms SIP1, SIP1-delta, 1700012N19Rik, Sip1, sip1, sip1-delta, SMN2
»Alternatives Human, Xenopus laevis
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
»Alternatives ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN112054
Quantity 0.1 mL
643.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen His-Gemin2 (starting at 2nd methionine)
Clone 2E17
Isotype IgG1
Specificity The antibody detects Gemin-2.
Purification Protein A chromatography.
Alternative Name SIP1 / GEMIN2
Background Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
Alternate names: Component of gems 2, GEMIN-2, SMN-interacting protein 1, Survival of motor neuron protein-interacting protein 1
NCBI Accession 9606
UniProt O14893
Application Notes ELISA. Immunofluorescence. Immunoprecipitation. Western blot (detects a band of approximately 34 kDa). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Immunofluorescence protocol - Formaldehyde fixationCollect cells from T. c. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperatureon an orbital shaker.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS with 0.09% sodium azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Background publications Liu, Fischer, Wang et al.: "The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins." in: Cell, Vol. 90, Issue 6, pp. 1013-21, 1997 (PubMed).

Alternatives for antigen "Gem (Nuclear Organelle) Associated Protein 2 (GEMIN2)", type "Antibodies"
Hosts (6), (6)
Reactivities (10), (5), (4), (3)
Applications (12), (8), (6), (6), (5), (4), (2), (1), (1)
Epitopes (2), (1), (1)