Gem (Nuclear Organelle) Associated Protein 2 (GEMIN2) antibody

Details for Product No. ABIN112054
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Antigen
Synonyms sip1, sip1-delta, SIP1, SIP1-delta, 1700012N19Rik, Sip1, HSPC082, SIP-1, SMADIP1, ZFHX1B
Reactivity
Human, Xenopus laevis
(61), (47), (42), (13), (12), (12), (8), (4)
Host
Mouse
(56), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(45), (21), (20), (12), (10), (9), (8), (7), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN112054
643.50 $
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Immunogen His-Gemin2 (starting at 2nd methionine)
Clone 2E17
Isotype IgG1
Specificity The antibody detects Gemin-2.
Purification Protein A chromatography.
Alternative Name SIP1 / GEMIN2
Background Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
Alternate names: Component of gems 2, GEMIN-2, SMN-interacting protein 1, Survival of motor neuron protein-interacting protein 1
Gene ID 9606
UniProt O14893
Application Notes ELISA. Immunofluorescence. Immunoprecipitation. Western blot (detects a band of approximately 34 kDa). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Immunofluorescence protocol - Formaldehyde fixationCollect cells from T. c. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperatureon an orbital shaker.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS with 0.09% sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Background publications Liu, Fischer, Wang et al.: "The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins." in: Cell, Vol. 90, Issue 6, pp. 1013-21, 1997 (PubMed).

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