Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) antibody

Details for Product No. ABIN112056
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Antigen
Synonyms hnrpa1, TPT1P, HNRPA1, HNRNPA1, hnRNP A1, HNRPA1L3, hnRNP-A1, D15Ertd119e, Hdp, Hnrpa1, hnrnp-A1, ROA1
Reactivity
Human, Mouse (Murine)
(65), (26), (24), (5), (3), (2), (1), (1), (1)
Host
Mouse
(50), (18)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(68), (45), (31), (27), (18), (15), (8), (2), (1)
Pubmed 3 references available
Quantity 0.1 mL
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Catalog No. ABIN112056
643.50 $
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Immunogen HeLa hnRNP-A1 (partially purified).
Clone 4B10
Isotype IgG2a
Specificity This antibody recognises hnRNP-A1.
Purification Protein A Chromatography.
Alternative Name hnRNP core protein A1 / HNRNPA1
Background This protein belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The HNRNP proteins have distinct nucleic acid binding properties. HNRNP A1 has two repeats of quasi RRM domains that bind to RNAs. It is one of the most abundant core proteins of HNRNP complexes and it is localized to the nucleoplasm. This protein, along with other HNRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre mRNA into HNRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought to have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for the HNRNP A1 gene but only two transcripts are fully described. These variant have multiple alternative transcription initiation sites and multiple polyA sites.
Alternate names: HNRPA1, Helix-destabilizing protein, Heterogeneous nuclear ribonucleoprotein A1, Single- strand RNA-binding protein, hnRNP-A1
Gene ID 9606
UniProt P09651
Application Notes ELISA. Western blot: Detects a band of approximately 34kDa. Immunoprecipitation. Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Immunofluorescence Protocol - Formaldehyde FixationCollect cells from T. c. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperatureon an orbital shaker. Remove PFA and incubate in 0. 5% Triton X-IOO in 1x PBS for 5 minutes at roomtemperature. Prepare blocking reagent, this is also the antibody diluent. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature. Prepare primary antibodies (50 µl/coverslip) and moist staining chambers. Wash cells 2x with lx PBS at room temperature and air dry briefly. Incubate with primary antibody for 1 hr at room temperature in the dark in stainingchambers. During this time prepare the secondary antibody. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker). Incubate with secondary antibody for 1 hour at room temperature in the dark in stainingchambers. Wash cells 5x with 1x PBS. Mount in Dapi. Solutions (prepare fresh the same day of staining). 1x Phosphate buffered saline. Blocking reagent: 1% NCS in 1x PBS (use fresh l0x PBS). Fixation solution: 3. 5% Para formaldehyde. 1. 75g PFA in 20 ml d. H20 plus 5 drops 1M NaOH. Stir on a hot plate at 50-60°C untildissolved. Add 4 drops IN HCI and check pH indicator strip. PH should be 7. 4. Completevolume with d. H20 to 25 ml and add 25 ml 2xPBS. Check pH before adding to cover slips. Immunofluorescence Protocol - Methanol/Acetone FixationCollect cells from T. C. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Fix cells with cold methanol: acetone 1: 1 for 10 minutes on ice. Prepare blocking reagent, this is also the diluent for the antibodies. Remove fixative and wash cells 3x with Ix PBS at RT, for 4 minutes/wash on orbital shaker. Block with 1% NCS and Ix PBS for 30 minutes at RT. Prepare primary antibodies (50 µl/coverslip) and moist staining chambers. Wash cells 2x with 1 x PBS at RT and air dry for approximately 7 minutes. Incubate with primary antibody for 1 hr at RT in the dark in staining chambers. During thistime prepare secondary antibody.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Piñol-Roma, Choi, Matunis et al.: "Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins." in: Genes & development, Vol. 2, Issue 2, pp. 215-27, 1988 (PubMed).

Kamma, Portman, Dreyfuss: "Cell type-specific expression of hnRNP proteins." in: Experimental cell research, Vol. 221, Issue 1, pp. 187-96, 1995 (PubMed).

Siomi, Eder, Kataoka et al.: "Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins." in: The Journal of cell biology, Vol. 138, Issue 6, pp. 1181-92, 1997 (PubMed).

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