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Epidermal Cytokeratin antibody

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Clonality (Clone)
Monoclonal ()
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 5 references available
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Quantity 0.1 mL
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Immunogen Human epidermal keratins.
Clone AE20
Isotype IgG
Specificity Human keratin K10-1 (56.5 Kda).
Purification Protein G chromatography.
Background This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
Application Notes Suitable for Immunblotting (1/1000-1/3000 for Western blot) and Immunohistochemicalstaining of Paraffin Sections (10 µg/ml). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Immunofluorescence protocol - Formaldehyde fixationCollect cells from T. c. unit and remove media from petri dish using suction.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.09% sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Background publications Novák, Radnai, Gorzó et al.: "Prevention of preterm delivery with periodontal treatment." in: Fetal diagnosis and therapy, Vol. 25, Issue 2, pp. 230-3, 2009 (PubMed).

Woodcock-Mitchell, Eichner, Nelson et al.: "Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies." in: The Journal of cell biology, Vol. 95, Issue 2 Pt 1, pp. 580-8, 1983 (PubMed).

Tseng, Jarvinen, Nelson et al.: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983 (PubMed).

Cooper, Schermer, Sun: "Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 52, Issue 3, pp. 243-56, 1985 (PubMed).

Loomis, Kolega, Manabe et al.: "Characterization of a keratinocyte-specific extracellular epitope of desmoglein. Implications for desmoglein heterogeneity and function." in: The Journal of biological chemistry, Vol. 267, Issue 23, pp. 16676-84, 1992 (PubMed).

Catalog No. ABIN112082

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