Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A) (pSer5) antibody

Details for Product No. ABIN112084
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms NCU02103.1, 19.m02399, POLR2A, 220kDa, Rpb1, Rpo2-1, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
Epitope
pSer5
(11), (11), (9), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Yeast
(55), (21), (20), (2), (1), (1), (1), (1)
Host
Mouse
(40), (18), (1), (1)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(54), (29), (25), (20), (14), (8), (6), (4), (3), (3), (1)
Pubmed 1 reference available
Quantity 50 µL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN112084
544.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide: YSPTSpPS (Human)
Clone 8A7
Isotype IgM
Specificity Recognises the phosphorylated (Ser5) form of RNA polymerase II in Human and S. cerevisiae.
Purification Protein A chromatography.
Alternative Name POLR2A
Background RNA polymerase II carboxy-terminal domain (CTD) interacts with a large multisubunit complex that contains TATA-binding protein (TBP) and is an integral part of the transcription initiation complex. Phosphorylation of RNA polymerase II's largest subunit C- terminal domain (CTD) is a key event during mRNA metabolism. Numerous enzymes, including cell cycle-dependent kinases and TFIIF-dependent phosphatases target the CTD.
Alternate names: DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit RPB1, POLR2, POLRA, RNA polymerase II, RNA polymerase II 220 kd, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB220, RPOL2
Gene ID 5430
NCBI Accession NP_000928
UniProt P24928
Application Notes Suitable for Western blot, ELISA and Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Immunofluorescence protocol - Formaldehyde fixationCollect cells from T. c. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperature
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.09% sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Otero, Fellows, Li et al.: "Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation." in: Molecular cell, Vol. 3, Issue 1, pp. 109-18, 1999 (PubMed).

Validation Images
Did you look for something else?
back to top