Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A) (pSer5) antibody

Antigen: Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A)

Synonyms: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220, MGC75453, Rpb1, 220kDa, Rpo2-1, 19.m02399, POLR2A

Binding Site: pSer5

Clonality: Monoclonal (8A7)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Western Blotting (WB), ELISA

Catalog no.: ABIN112084

Additional Information

Alternative name: POLR2A

Gene ID: 5430

UniProt: P24928

Immunogen: Synthetic peptide: YSPTSpPS (Human)

Cross-Reactivity: Human, Yeast

Isotype: IgM

Clone: 8A7

Description: RNA polymerase II carboxy-terminal domain (CTD) interacts with a large multisubunitcomplex that contains TATA-binding protein (TBP) and is an integral part of thetranscription initiation complex. Phosphorylation of RNA polymerase II's largest subunitC-terminal domain (CTD) is a key event during mRNA metabolism. Numerous enzymes,including cell cycle-dependent kinases and TFIIF-dependent phosphatases target the CTD.

Characteristics: Synonyms: RNA polymerase II subunit B1, POLR2, POLRA, RPOL2, RPB1, RPB220, DNA-directed RNApolymerase II subunit RPB1, RNA-directed RNA polymerase II subunit RPB1, DNA-directedRNA polymerase II largest subunit, RNA polymerase II 220 kd

Specificity: Recognises the phosphorylated (Ser5) form of RNA polymerase II in Human and S. cerevisiae.

Application Details

Protocol: Immunofluorescence protocol - Formaldehyde fixationCollect cells from T. c. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperature

Application Notes: Suitable for Western blot, ELISA and Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS with 0.09% sodium azide as preservative.

Storage: Store the antibody at 2-8°C for one month or (in small aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only

Publications

Otero, Fellows, Li et al.: "Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation." in: Molecular cell, Vol. 3, Issue 1, pp. 109-18, 1999 (PubMed).