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Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A) (pSer5) antibody

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(9), (7), (6), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Human, Yeast
(55), (23), (23), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(43), (12), (2), (1)
Clonality (Clone)
Monoclonal ()
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(51), (24), (23), (23), (10), (6), (5), (3), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μL
Shipping to United States ( )
Immunogen Synthetic peptide: YSPTSpPS (Human)
Clone 8A7
Isotype IgM
Specificity Recognises the phosphorylated (Ser5) form of RNA polymerase II in Human and S. cerevisiae.
Purification Protein A chromatography.
Alternative Name POLR2A (POLR2A Antibody Abstract)
Background RNA polymerase II carboxy-terminal domain (CTD) interacts with a large multisubunit complex that contains TATA-binding protein (TBP) and is an integral part of the transcription initiation complex. Phosphorylation of RNA polymerase II's largest subunit C- terminal domain (CTD) is a key event during mRNA metabolism. Numerous enzymes, including cell cycle-dependent kinases and TFIIF-dependent phosphatases target the CTD.
Alternate names: DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit RPB1, POLR2, POLRA, RNA polymerase II, RNA polymerase II 220 kd, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB220, RPOL2
Gene ID 5430
NCBI Accession NP_000928
UniProt P24928
Pathways Regulatory RNA Pathways
Application Notes Suitable for Western blot, ELISA and Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Immunofluorescence protocol - Formaldehyde fixationCollect cells from T. c. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperature
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.09% sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Otero, Fellows, Li et al.: "Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation." in: Molecular cell, Vol. 3, Issue 1, pp. 109-18, 1999 (PubMed).

Catalog No. ABIN112084

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