Adeno-Associated Virus 2 (AAV-2) (intact particle) antibody

Antigen: Adeno-Associated Virus 2 (AAV-2) (intact particle)

Clonality: Monoclonal (A20)

Host: Mouse

Reactivity: Human, Monkey

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN112130

Additional Information

Characteristics: Synonyms: AAV2

Immunogen: Adeno-associated virus capsid proteins and virus particles

Isotype: IgG3  (Matching secondary antibodies)

Clone: A20

Specificity: For characterization of different stages of infection and very useful for the analysis of theAAV assembly process. A20 specifically reacts with intact adeno-associated virus particles,empty and full capsids. Recognizes a conformational epitope of assembled capsids, notpresent in denatured capsid proteins and native but unassembled capsid proteins. Theantibody cannot be used for immunoblotting. Epitope mapping experiments (Wobus et al. ,see below) identified four immunoreactive (discontinous) regions. The major reaction wasattributed to sequence aa 369 to aa378 of AAV-2 capsids. The antibody is also useful forneutralizing experiments (cf. Moskalenko et. al).

Application Details

Application Notes: Immunofluorescence microscopy: 1: 20, Overnight at 2-8C. Immunohistochemistry: Overnight at 2-8C. Immunoprecipitation: 1: 5. Neutralization assay. Affinity chromatography. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS or Tris-buffered saline contains 0.09% sodium azide and 0.5% BSAReconstitution: Restore with 1 ml dist. water

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Publications: Wistuba, Weger, Kern et al.: "Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins." in: Journal of virology, Vol. 69, Issue 9, pp. 5311-9, 1995 (PubMed).

Wistuba, Kern, Weger et al.: "Subcellular compartmentalization of adeno-associated virus type 2 assembly." in: Journal of virology, Vol. 71, Issue 2, pp. 1341-52, 1997 (PubMed).

Grimm, Kern, Pawlita et al.: "Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2." in: Gene therapy, Vol. 6, Issue 7, pp. 1322-30, 2000 (PubMed).

Moskalenko, Chen, van Roey et al.: "Epitope mapping of human anti-adeno-associated virus type 2 neutralizing antibodies: implications for gene therapy and virus structure." in: Journal of virology, Vol. 74, Issue 4, pp. 1761-6, 2000 (PubMed).

Wobus, Huegle-Doerr, Girod et al.: "Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection." in: Journal of virology, Vol. 74, Issue 19, pp. 9281-93, 2000 (PubMed).