Cyclin-Dependent Kinase 7 (CDK7) antibody

Antigen: Cyclin-Dependent Kinase 7 (CDK7)

Synonyms: CAK, p34, p37, CAK1, MO15, STK1, CDKN7, p39MO15, Crk4, Cdkn7, AI323415, AI528512, C230069N13, CDK7, DmCDK7, DmMO15, cdk-7, dCdk7, p40[MO15], DmelCG3319, CG3319, MGC85821, zgc:85821, MGC140417, cak1, stk1, cdkn7, p39mo15, p40MO15

Clonality: Monoclonal (MO1-1)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN112141

Additional Information

Alternative name: CDK7

UniProt: P50613

Immunogen: Recombinant 221 amino acid fragment of the c-terminus of human p40MO15.

Cross-Reactivity: Human, Rat (Rattus)

Isotype: IgG2b

Clone: MO1-1

Description: CDK7 is cdk activating kinase associated with the 34kDa cyclin H protein, and a 36kDaunnamed protein. CDK7/cyclin H is an integral part of the general transcription factorcomplex TFIIH, hence is important for activated transcription of polymerase II-dependentpromoters and DNA repair. CDK7 is nuclear at all stages of the cell division cycle, except formitosis indicating that its activity is required for key CDKs operating in distinct cell cyclephases.

Characteristics: Synonyms: CAK1, P39 Mo15, STK1, CDK-activating kinase, Cell division protein kinase 7, TFIIH basaltranscription factor complex kinase subunit, 39 kDa protein kinase

Synonyms: CAK, p34, p37, CAK1, MO15, STK1, CDKN7, p39MO15, Crk4, Cdkn7, AI323415, AI528512, C230069N13, CDK7, DmCDK7, DmMO15, cdk-7, dCdk7, p40[MO15], DmelCG3319, CG3319, MGC85821, zgc:85821, MGC140417, cak1, stk1, cdkn7, p39mo15, p40MO15

Application Details

Application Notes: Immunohistochemistry on frozen sections: dilute 1: 20-1: 40 in an ABC method (60 minutesat room temperature). Also useful for Western blotting, Immunocytochemistry and Flow Cytometry. Positive Control: TonsilOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Buffer: Contains 0.09% sodium azide as preservative

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cell Cycle

Restrictions: For Research Use only

Images

Paraformaldehyde-fixed human cervical cancer cells (HeLa) stained cdk7 Ab (Cat. BM5019) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cultured cells

Publications

Tassan, Schultz, Bartek et al.: "Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase)." in: The Journal of cell biology, Vol. 127, Issue 2, pp. 467-78, 1994 (PubMed).