Keratin 8 (KRT8) antibody

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Antigen
  • ck8
  • cyk8
  • k2c8
  • card2
  • krt2-5
  • MGC69490
  • KRT8
  • DKFZp468F2127
  • CARD2
  • CK-8
  • CK8
  • CYK8
  • K2C8
  • K8
  • KO
  • KRT2-8
  • AA960620
  • AL022697
  • AU019895
  • Card2
  • EndoA
  • Krt-2.8
  • Krt2-8
  • CYKER
  • DreK8
  • cb186
  • krt2-8
  • sb:cb186
  • wu:fa20h05
  • wu:fa95h10
  • wu:fb96c06
  • zf-K8
  • zfk8
  • KERATIN8
  • keratin 8
  • krt8
  • KRT8
  • Krt8
Reactivity
Human, Mammalian, Mouse (Murine), Rabbit, Rat (Rattus)
778
173
138
63
61
50
42
38
21
20
16
14
10
6
5
3
2
2
1
1
1
1
Host
Mouse
550
238
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
32
21
15
15
14
14
13
12
12
12
12
12
12
12
12
10
10
3
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
593
408
329
252
231
229
227
226
98
28
17
16
14
13
12
6
2
2
1
1
Options
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Immunogen Keratin isolated from the Human breast carcinoma cell line MCF-7
Clone M20
Isotype IgG1
Specificity M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells. The M20 epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of Cytokeratin 8 (See Reference 10).
Purification Purified
Alternative Name Cytokeratin 8 (KRT8 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8
Gene ID 3856
NCBI Accession NP_002264
UniProt P05787
Research Area Cancer, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Immunoblotting: 1/100-1/1000Immunocytochemistry. Flow Cytometry. Immunohistochemistry on Frozen Tissues: 1/100-1/200 with Avidin-biotinylatedHorseradish peroxidase complex ABC as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.
Background publications Lanyi, Schobert: "Local-global conformational coupling in a heptahelical membrane protein: transport mechanism from crystal structures of the nine states in the bacteriorhodopsin photocycle." in: Biochemistry, Vol. 43, Issue 1, pp. 3-8, 2004 (PubMed).

Ku, Gish, Wright, Omary: "Keratin 8 mutations in patients with cryptogenic liver disease." in: The New England journal of medicine, Vol. 344, Issue 21, pp. 1580-7, 2001 (PubMed).

Wetzels, Kuijpers, Lane, Leigh, Troyanovsky, Holland, van Haelst, Ramaekers: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." in: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, 1991 (PubMed).

Pecoraro, Lee, Morgan, Defendi: "Evolution of in vitro transformation and tumorigenesis of HPV16 and HPV18 immortalized primary cervical epithelial cells." in: The American journal of pathology, Vol. 138, Issue 1, pp. 1-8, 1991 (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Schaafsma, Ramaekers, van Muijen, Lane, Leigh, Robben, Huijsmans, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Ivanyi, Groeneveld, Van Doornewaard, Mooi, Hageman: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." in: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).

Schaafsma, Ramaekers, van Muijen, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract." in: Histochemistry, Vol. 91, Issue 2, pp. 151-9, 1989 (PubMed).

Kawakami, Hirano: "Distribution of glycoconjugates on the plasma membrane and on membranes of the open-canalicular system in human platelets. A cytochemical study." in: Histochemistry, Vol. 91, Issue 1, pp. 1-4, 1989 (PubMed).

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987 (PubMed).

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987 (PubMed).

Odin, Obrink: "Quantitative determination of the organ distribution of the cell adhesion molecule cell-CAM 105 by radioimmunoassay." in: Experimental cell research, Vol. 171, Issue 1, pp. 1-15, 1987 (PubMed).