Keratin 9 (KRT9) (N-Term), (AA 4-28) antibody

Details for Product No. ABIN112174
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Antigen
Synonyms CK-9, EPPK, K9, Krt1-9
Epitope
N-Term, AA 4-28
(7), (7), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(29), (1), (1), (1), (1), (1)
Host
Mouse
(16), (10), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(27), (13), (11), (10), (9), (7), (4), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 1 mL
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Catalog No. ABIN112174
401.50 $
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Immunogen Synthetic peptides [amino acids pos. 450-477 / N-terminal amino acids nos. 4-28] of human cytokeratin 9 (MW 62,129)
Clone Ks9-70-Ks9-216
Isotype Cocktail
Specificity The antibody cocktail is an excellent tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment. The antibody reagent represents an excellent marker to study palmoplantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation- promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum.
Purification Supernatant
Alternative Name Cytokeratin 9
Background Keratin 9 is a type I cytokeratin. It is expressed in the terminally differentiated epidermis of palms and soles. Defects in KRT9 are a cause of epidermolytic palmoplantar keratoderma (EPPK). EPPK is an autosomal dominant disease characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins.
Alternate names: CK9, Cytokeratin 9, K9, KRT9, Keratin, Keratin-9, Spermatid Perinuclear Ring Manchette Protein K9, Type I cytoskeletal 9
Gene ID 3857
NCBI Accession NP_000217
UniProt P35527
Research Area Signaling, Extracellular Matrix
Application Notes Immunohistochemistry on frozen and Paraffin embedded tissues (1: 20, PAP/ABC, forParaffin sections, microwave treatment and in some cases protease digestion isrecommended). Immunofluorescence (1: 10). Western blotting (1/25-1/300). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions
Expiry Date 12 months
Background publications Langbein, Heid, Moll et al.: "Molecular characterization of the body site-specific human epidermal cytokeratin 9: cDNA cloning, amino acid sequence, and tissue specificity of gene expression." in: Differentiation; research in biological diversity, Vol. 55, Issue 1, pp. 57-71, 1994 (PubMed).

Swensson, Langbein, McMillan et al.: "Specialized keratin expression pattern in human ridged skin as an adaptation to high physical stress." in: The British journal of dermatology, Vol. 139, Issue 5, pp. 767-75, 1999 (PubMed).

Mochida, Rivkin, Gil et al.: "Keratin 9 is a component of the perinuclear ring of the manchette of rat spermatids." in: Developmental biology, Vol. 227, Issue 2, pp. 510-9, 2000 (PubMed).

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