Keratin 9 (KRT9) antibody

Antigen: Keratin 9 (KRT9)

Synonyms: K9, CK-9, EPPK, Krt1-9

Clonality: Monoclonal (Ks9-70-Ks9-216)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN112174

Additional Information

Alternative name: Cytokeratin 9

UniProt: P35527

Immunogen: Synthetic peptides [amino acids pos. 450-477 / N-terminal amino acids nos. 4-28] ofhuman cytokeratin 9 (MW 62,129)

Cross-Reactivity: Human

Isotype: Cocktail

Clone: Ks9-70-Ks9-216

Description: Keratin 9 is a type I cytokeratin. It is expressed in the terminally differentiated epidermis ofpalms and soles. Defects in KRT9 are a cause of epidermolytic palmoplantar keratoderma(EPPK). EPPK is an autosomal dominant disease characterized by diffuse thickening of theepidermis on the entire surface of palms and soles sharply bordered with erythematousmargins.

Characteristics: Synonyms: Cytokeratin 9, KRT9, CK9, Keratin-9, K9, Keratin, Type I cytoskeletal 9, SpermatidPerinuclear Ring Manchette Protein K9

Application Details

Application Notes: Immunohistochemistry on frozen and Paraffin embedded tissues (1: 20, PAP/ABC, forParaffin sections, microwave treatment and in some cases protease digestion isrecommended). Immunofluorescence (1: 10). Western blotting (1/25-1/300). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Storage: Store the antibody at 2-8°C. Shelf life: one year from despatch.

Research Area: Signaling, Extracellular Matrix

Restrictions: For Research Use only

Publications

Langbein, Heid, Moll et al.: "Molecular characterization of the body site-specific human epidermal cytokeratin 9: cDNA cloning, amino acid sequence, and tissue specificity of gene expression." in: Differentiation; research in biological diversity, Vol. 55, Issue 1, pp. 57-71, 1994 (PubMed).

Swensson, Langbein, McMillan et al.: "Specialized keratin expression pattern in human ridged skin as an adaptation to high physical stress." in: The British journal of dermatology, Vol. 139, Issue 5, pp. 767-75, 1999 (PubMed).

Mochida, Rivkin, Gil et al.: "Keratin 9 is a component of the perinuclear ring of the manchette of rat spermatids." in: Developmental biology, Vol. 227, Issue 2, pp. 510-9, 2000 (PubMed).