U1 SnRNP Protein A (0) antibody

Antigen: U1 SnRNP Protein A (0)

Synonyms: U1A, Rnu1a1, Rnu1a-1, C430021M15Rik, SNRPA, MGC137110

Clonality: Monoclonal (9A9)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN112211

Additional Information

Characteristics: Synonyms: U1A protein, U1-A, SNRPA, U1 small nuclear ribonucleoprotein A

Alternative name: U1 snRNP protein A

Swiss-Prot: P09012

Immunogen: Recombinant beta-galactosidase U2B'' fusion protein

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9A9

Specificity: The mab reacts primarily with the human U1A protein and has a minor cross reactivity withthe U2B'' protein, contained in the U1 and U2 small nuclear ribonucleoprotein (snRNP)complexes. Species: Human. Others not tested.

Application Details

Application Notes: Immunoprecipitation. Immunofluorescence microscopy: 1: 5 - 1: 10. Recommended Positive Control: HEp-2 cells. Immunoblotting: 1: 5 - 1: 10. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 6 µg/ml

Buffer: PBS containing 1% BSA and 0.1% NaN3 (reconstituted)Reconstitution: Restore with 1 ml PBS

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Publications: Habets, Hoet, De Jong et al.: "Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally-induced mouse monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 8, pp. 2560-6, 1989 (PubMed).