U2 SnRNP Protein B2 antibody

Antigen: U2 SnRNP Protein B2

Clonality: Monoclonal (4G3)

Host: Mouse

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN112212

Additional Information

Characteristics: Synonyms: U2 snRNP B'', SNRPB2, U2 small nuclear ribonucleoprotein B

Swiss-Prot: P08579

Immunogen: Recombinant beta-galactosidase U2B'' fusion protein.

Format: Supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 4G3

Description: The protein encoded by this gene associates with stem loop IV of U2 small nuclearribonucleoprotein (U2 snRNP) in the presence of snRNP-A'. The encoded protein may play arole in pre-mRNA splicing. Autoantibodies from patients with systemic lupuserythematosus frequently recognize epitopes on the encoded protein. Two transcriptvariants encoding the same protein have been found for this gene. Transcript Variant: Thisvariant differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the sameprotein. Publication Note: This RefSeq record includes a subset of the publications that areavailable for this gene. Please see the Entrez Gene record to access additionalpublications.

Specificity: The mab reacts primarily with the U2B'' protein from a variety of species ranging fromhuman to plant. The monoclonal antibody does not react detectably with the veryhomologous U1A protein.

Application Details

Application Notes: Immunofluorescence Microscopy: 1/5-1/10. Recommended Positive Control: HEp-2 cells. Immunoprecipitation. Immunoblotting: 1/5-1/10. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: Final Solution contains: PBS with 1% BSA as stabilizer and and 0.09%Sodium Azide as preservative.Reconstitution: Restore with 1 ml PBS.

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Publications: Simpson, Vaux, Clark et al.: "Evolutionary conservation of the spliceosomal protein, U2B''." in: Nucleic acids research, Vol. 19, Issue 19, pp. 5213-7, 1991 (PubMed).

Habets, Hoet, De Jong et al.: "Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally-induced mouse monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 8, pp. 2560-6, 1989 (PubMed).