Insulin antibody

Catalog No. ABIN112214

Product Details anti-Insulin Antibody

Target: Insulin (INS)

Reactivity: Human, Pig, Mouse, Cow, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes both Insulin and Proinsulin, but does not react with free C-Peptide.Aff. Const.:6.3 x 108 M-1

Cross-Reactivity (Details): Also reacts to: Proinsulin

Purification: Affinity Chromatography on Protein A

Immunogen: Recombinant Human Insulin.

Clone: D3E7 (5B6-6)

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections. ELISA: This antibody is suitable for use as a Detection antibody with BM507 as the Captureantibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin (INS Products)

Synonyms: IDDM2 antibody, ILPR antibody, IRDN antibody, MODY10 antibody, ins1 antibody, xins antibody, ins1-a antibody, Insulin antibody, AA986540 antibody, Ins-2 antibody, InsII antibody, Mody antibody, Mody4 antibody, proinsulin antibody, zgc:109842 antibody, igf2-A antibody, ins antibody, ins-a antibody, ins-b antibody, insulin antibody, insulin precursor antibody, insulin II antibody, preproinsulin antibody, insulin L homeolog antibody, insulin S homeolog antibody, INS antibody, INS-IGF2 antibody, ins antibody, Ins antibody, PIN antibody, Ins2 antibody, ins.L antibody, ins.S antibody

Background: Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS

Gene ID: 3630

NCBI Accession: NP_000198

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation