Smoothelin (SMTN) antibody

Details for Product No. ABIN112216
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Antigen
Synonyms MGC68765, SMTN, MGC140000, MGC155237, DKFZp468D019, smsmo
Reactivity
Dog (Canine), Chicken, Cat (Feline), Human, Monkey, Pig (Porcine), Rat (Rattus)
(9), (5), (3), (3), (3), (2), (1), (1)
Host
Mouse
(12)
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(11), (10), (8), (4), (3), (3), (1)
Pubmed 6 references available
Quantity 0.1 mL
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Catalog No. ABIN112216
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Immunogen Cytoskeletal extract of Chicken gizzard
Clone R4A
Isotype IgG1
Specificity This antibody is specific for Smoothelin. Reactive with a novel cytoskeletal protein specific for smooth muscle cells: smoothelin. In Western Blot 2 Molecular weight isoforms are detected: 59 kDa isoform specific to visceral SMC (Smoothelin A) and 100 kDa isoform in vascular SMC (Smoothelin B). The antibody is very useful for characterization of atherosclerotic lesions. The antibody is useful tool to monitor SMC differentiation. Confocal scanning laser microscopy of tissue sections and cells in culture showed a filamentous organization of smoothelin co-localizing with actin stress fibers.
Cross-Reactivity (Details) Species reactivity (tested):Human, Monkey, Canine, Feline, Porcine and Chicken
Purification Purified
Alternative Name Smoothelin
Background Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is located on chromosome 22.Synonyms: SMSMO, SMTN
Gene ID 6525, 9606
UniProt P53814
Research Area Organogenesis, Cytoskeleton
Application Notes Immunoblotting. Cytological Material. Immunofluorescence Microscopy. Immunohistochemistry on Frozen and Paraffin Embedded Tissue (Microwave pretreatmentis required). Working Duilution: Dilute 1/100-1/200 with PBS ( pH 7.4) for Immunohistochemicalapplication (ABC method) and 1/1000 for Western Blotting (ECL method). Incubation Time: 1 h at RT, extended with Paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications van der Loop, Schaart, Timmer et al.: "Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells." in: The Journal of cell biology, Vol. 134, Issue 2, pp. 401-11, 1996 (PubMed).

Wehrens, Mies, Gimona et al.: "Localization of smoothelin in avian smooth muscle and identification of a vascular-specific isoform." in: FEBS letters, Vol. 405, Issue 3, pp. 315-20, 1997 (PubMed).

van der Loop, Gabbiani, Kohnen et al.: "Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 17, Issue 4, pp. 665-71, 1997 (PubMed).

Johansson, Eriksson, Ramaekers et al.: "Smoothelin in adult and developing human arteries and myocardium." in: Histochemistry and cell biology, Vol. 112, Issue 4, pp. 291-9, 2000 (PubMed).

Johansson, Eriksson, Ramaekers et al.: "Smoothelin and intermediate filament proteins in human aortocoronary saphenous vein by-pass grafts." in: The Histochemical journal, Vol. 31, Issue 11, pp. 723-7, 2000 (PubMed).

Deruiter, Rensen, Coolen et al.: "Smoothelin expression during chicken embryogenesis: detection of an embryonic isoform." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 221, Issue 4, pp. 460-3, 2001 (PubMed).

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