Smoothelin (SMTN) antibody

Antigen: Smoothelin (SMTN)

Synonyms: FLJ35365, FLJ38597, smsmo, MGC68765, SMTN, MGC140000, MGC155237, DKFZp468D019

Clonality: Monoclonal (R4A)

Host: Mouse

Reactivity: Dog (Canine), Chicken, Cat (Feline), Human, Monkey, Pig (Porcine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN112216

Additional Information

Alternative name: Smoothelin

Gene ID: 6525

UniProt: P53814

Immunogen: Cytoskeletal extract of Chicken gizzard.

Cross-Reactivity: Dog (Canine), Chicken, Cat (Feline), Human, Monkey, Pig (Porcine), Rat (Rattus)

Isotype: IgG1

Clone: R4A

Description: Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodiesdirected to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin isexclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analysesreveal a broad species distribution of this protein. Cells with SMC like characteristics, suchas myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not containsmoothelin. Human Smoothelin is encoded by a single copy of gene located on chromosome 22.

Characteristics: Synonyms: SMTN, SMSMO

Application Details

Application Notes: Immunoblotting. Cytological Material. Immunofluorescence Microscopy. Immunohistochemistry on Frozen and Paraffin Embedded Tissue (Microwave pretreatmentis required). Working Duilution: Dilute 1/100-1/200 with PBS (pH 7.4) for Immunohistochemicalapplication (ABC method) and 1/1000 for Western Blotting (ECL method). Incubation Time: 1 h at RT, extended with Paraffin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS with 0.09% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Organogenesis, Cytoskeleton

Restrictions: For Research Use only

Publications

van der Loop, Schaart, Timmer et al.: "Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells." in: The Journal of cell biology, Vol. 134, Issue 2, pp. 401-11, 1996 (PubMed).

Wehrens, Mies, Gimona et al.: "Localization of smoothelin in avian smooth muscle and identification of a vascular-specific isoform." in: FEBS letters, Vol. 405, Issue 3, pp. 315-20, 1997 (PubMed).

van der Loop, Gabbiani, Kohnen et al.: "Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 17, Issue 4, pp. 665-71, 1997 (PubMed).