Keratin 2 (KRT2) (N-Term), (AA 2-23) antibody

Details for Product No. ABIN112226
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Antigen
Synonyms CK-2e, K2e, KRT2A, KRT2E, KRTE, Kb2, K2E, Krt2e, Krt2-2, Krt2-17, BB005427, KRT2, krt2a
Epitope
N-Term, AA 2-23
(6), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(21), (8), (5), (3), (2), (1), (1)
Host
Mouse
(15), (13), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(29), (16), (14), (12), (8), (7), (4), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN112226
Quantity 1 mL
Price
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Immunogen Synthetic peptide (N-terminal amino acids nos. 2-23) of Human Cytokeratin K2 (MW 65,852).
Clone Ks 2-342-7-4
Isotype IgG1
Specificity Ks 2.342.7.4 represents an excellent marker to study terminal epidermal differentiation. The Monoclonal antibody is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K10). It is also reactive on hyperkeratosis of diverse viral and genetic origin. The Mab does not react with palate keratin K76. Reactive Polypetide: Basic human keratin K2 (MW 65,852, formerly also designated Cytokeratin 2e).
Purification Supernatant
Alternative Name Cytokeratin 2e
Background Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner.
Alternate names: CK2e, Cytokeratin-2e, K2e, KRT2, KRT2A, KRT2E, Keratin, Keratin-2, type II cytoskeletal 2 epidermal
NCBI Accession 9606
UniProt P35908
Research Area Extracellular Matrix
Application Notes Western Blot (Alkaline Phosphatase): 1/200Western Blot (ECL): 1/60.000Immunofluorescence: 1/200Immunohistochemistry on Frozen Sections: 1/100. Pretreatment with Triton-X100 isrecommended (e. g.0.2% in PBS for 5 min)Immunohistochemistry on Paraffin Sections: 1/100 (Microwave method is recommended). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.09% Sodium Azide
Preservative Sodium azide
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Collin, Moll, Kubicka et al.: "Characterization of human cytokeratin 2, an epidermal cytoskeletal protein synthesized late during differentiation." in: Experimental cell research, Vol. 202, Issue 1, pp. 132-41, 1992 (PubMed).

Swensson, Langbein, McMillan et al.: "Specialized keratin expression pattern in human ridged skin as an adaptation to high physical stress." in: The British journal of dermatology, Vol. 139, Issue 5, pp. 767-75, 1999 (PubMed).

Hosts (15), (13), (3)
Reactivities (21), (8), (5), (3), (2), (1), (1)
Applications (29), (16), (14), (12), (8), (7), (4), (1), (1), (1)
Conjugates (2)
Epitopes (6), (4), (2), (1), (1), (1), (1), (1), (1), (1)
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