Cadherin 11 antibody

Antigen: Cadherin 11

Synonyms: OB, CAD11, CDHOB, OSF-4, cad11, cdhob, osf-4, Xcad-11, MGC108014, OB-cadherin, cadherin-11, cdh11

Clonality: Monoclonal (1A6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN112231

Additional Information

Characteristics: Synonyms: Osteoblast cadherin, OSF-4, CDH11, OB Cadherin

Alternative name: Cadherin-11

Swiss-Prot: P55287

Immunogen: GST fusion protein of part of the extracellular domain of cadherin 11 (OB-Cadherin)

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 1A6

Description: The cadherin gene family encodes calcium-dependent adhesion molecules that promotehomophilic interactions among cells. All cadherins are single-pass transmembraneproteins with a variable number of extracellular domains (EC, 110 amino acids long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain andare divided in type I and type II subgroups. Type I comprises cadherin E, N, P, and R. Type IIinclude cadherin-5, -6, -8, -11, and -12. Cadherin-11 (OB-cadherin) is a marker of the loosely connected and migratory cellularelements of the mesenchyme. Strong expression of cadherin-11 has been found in brain,spinal cord, bone marrow and bone cells, and it is regulated in human endometrialglandular epithelial and stromal cells. Recently, cadherin-11 mRNA and protein were foundto be expressed in most invasive breast cancer cell lines tested (but not in non-invasivecell lines), suggesting cadherin-11 as a potential marker for aggressive, invasive tumorsubsets.

Specificity: Antibody BM5096 is reactive with Cadherin 11. Species: Human. Others not tested.

Application Details

Application Notes: Immunohistochemistry (suitable for frozen sections, paraformaldehyde fixed, with 0.2%Triton X-100 in PBS): neat - approx.1: 10. Dilute immediately before use with PBS. Incubation time: 1 h at room temperature / overnight at 2-8C. Recommended positive control: PC-3 cell line (human prostate cell line). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store undiluted at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Extracellular Matrix, Signaling, Cytoskeleton

Restrictions: For Research Use only

Publications

Publications: Bussemakers, Van Bokhoven, Tomita et al.: "Complex cadherin expression in human prostate cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 85, Issue 3, pp. 446-50, 2000 (PubMed).

Tomita, van Bokhoven, van Leenders et al.: "Cadherin switching in human prostate cancer progression." in: Cancer research, Vol. 60, Issue 13, pp. 3650-4, 2000 (PubMed).