Cadherin 6 antibody

Antigen: Cadherin 6

Synonyms: cad6, MGC129338, MGC129360, K-cadherin, cadherin-6

Clonality: Monoclonal (2B6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN112232

Additional Information

Characteristics: Synonyms: CDH6, Kidney cadherin, Cadherin K, K-Cadherin

Alternative name: Cadherin-6

Swiss-Prot: P55285

Immunogen: GST fusion protein of part of the extracellular domain of cadherin 6 (K-Cadherin).

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2B6

Description: The cadherin gene family encodes calcium-dependent adhesion molecules that promotehomophilic interactions among cells. All cadherins are single-pass transmembraneproteins with a variable number of extracellular domains (EC, 110 amino acids long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain andare divided in type I and type II subgroups. Type I comprises cadherin E, N, P, and R. Type IIinclude cadherin-5, -6, -8, -11, and -12. During embryogenesis differential expression ofcadherins can drive morphogenesis by stimulating cell aggregation, defining boundariesbetween groups of cells and promoting cell migration. Cadherin-6 seems to be required forthe early aggregation of induced mesenchymal cells and their subsequent conversion toepithelium.

Specificity: This antibody reacts to Cadherin 6. Species: Human. Others not tested.

Application Details

Application Notes: Immunohistochemistry on frozen sections: paraformaldehyde fixed, with 0.2% Triton X-100in PBS, Ready to use, 1 h at room temperature / overnight at 2-8C. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Extracellular Matrix, Signaling, Cytoskeleton

Restrictions: For Research Use only

Publications

Publications: Shimazui, Oosterwijk, Akaza et al.: "Expression of cadherin-6 as a novel diagnostic tool to predict prognosis of patients with E-cadherin-absent renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 4, Issue 10, pp. 2419-24, 1998 (PubMed).

Bussemakers, Van Bokhoven, Tomita et al.: "Complex cadherin expression in human prostate cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 85, Issue 3, pp. 446-50, 2000 (PubMed).