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Cingulin antibody (CGN) (AA 4-24)

Details for Product anti-CGN Antibody No. ABIN112233, Supplier: Log in to see
Antigen
  • 6330408J11Rik
  • AI647528
  • AI987749
  • cb117
  • CGN
  • cgn
  • im:7137721
  • sb:cb117
  • sb:cb117.
  • si:dkey-204a24.2
Epitope
AA 4-24
2
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Xenopus laevis
18
8
6
2
2
1
Host
Mouse
13
3
2
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
16
7
6
6
5
4
3
2
Supplier
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Immunogen Synthetic peptide (aa. 4-24)
Clone 03-03-39
Isotype IgM
Specificity This monoclonal antibody reacts specifically with Cingulin (a 150 kDa polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas), of transitional epithelia (e.g. bladder epithelium), and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina). Negative with endothelia of vessels and capillaries (e.g. in heart and brain). In contrast to Symplekin, using Monoclonal antibody 139.4 Cingulin has been localized exclusively to the tight junction complex. Tested Reactivities on Cultured Cell Lines: Carcinoma cell lines (e.g. MCF-7, Caco-2, PLC, A-431), SV80 (fibroblasts), Glioma, MDBK, MDCK, BMGE.
Cross-Reactivity (Details) Species reactivity (tested):Human, Bovine, Mouse and Xenopus laevis.Negative with Rabbit.
Purification Supernatant
Alternative Name Cingulin (CGN Antibody Abstract)
Background Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data), apparent Mr 150 kD (after SDS-PAGE), pI 6.5 (very similar to that of symplekin, another junctional protein).Synonyms: CGN, KIAA1319
Gene ID 57530
NCBI Accession NP_065821
UniProt Q9P2M7
Application Notes Immunoblotting. Immunohistochemistry on Frozen and Paraffin Sections (only after microwave treatment): Ready-to-use.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Langbein, Pape, Grund, Kuhn, Praetzel, Moll, Moll, Franke: "Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas." in: European journal of cell biology, Vol. 82, Issue 8, pp. 385-400, 2003 (PubMed).

Langbein, Grund, Kuhn, Praetzel, Kartenbeck, Brandner, Moll, Franke: "Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom." in: European journal of cell biology, Vol. 81, Issue 8, pp. 419-35, 2002 (PubMed).

Zahraoui, Louvard, Galli: "Tight junction, a platform for trafficking and signaling protein complexes." in: The Journal of cell biology, Vol. 151, Issue 5, pp. F31-6, 2000 (PubMed).

Keon, Schäfer, Kuhn, Grund, Franke: "Symplekin, a novel type of tight junction plaque protein." in: The Journal of cell biology, Vol. 134, Issue 4, pp. 1003-18, 1996 (PubMed).