Cingulin (CGN) antibody

Antigen: Cingulin (CGN)

Synonyms: CGN, AI647528, AI987749, MGC118157, 6330408J11Rik, FLJ39281, KIAA1319, DKFZp779N1112, LOC398149, cgn, MGC98860

Clonality: Monoclonal (139-3-4)

Host: Mouse

Reactivity: Cow (Bovine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN112233

Additional Information

Alternative name: Cingulin

UniProt: Q9P2M7

Immunogen: Synthetic peptide (aa. 4-24)

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Xenopus laevis

Isotype: IgM

Clone: 139-3-4

Description: Cingulin, 126. 5 kD polypeptide (calculated from aa sequence data), apparent Mr 150 kD(after SDS-PAGE), pI 6. 5 (very similar to symplekin, another junctional protein)

Characteristics: Synonyms: CGN, KIAA1319

Specificity: This monoclonal antibody reacts specifically with Cingulin (a 150 kD polypeptide) presentin tight junctions (zonula occludens) of polar epithelia (e. g. colon, duodenum, kidney,liver, pancreas), of transitional epithelia (e. g. bladder epithelium), and of stratifiedepithelia (e. g. skin, foot sole, tongue, oesophagus, vagina). Negative with endothelia of vessels and capillaries (e. g. in heart and brain). Species: Human, Bovine, Mouse and Xenopus laevis. Negative with Rabbit. Others not tested.

Application Details

Application Notes: Immunoblotting. Immunohistochemistry on Frozen and Paraffin Sections (only after microwave treatment): Ready-to-use. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Keon, Schäfer, Kuhn et al.: "Symplekin, a novel type of tight junction plaque protein." in: The Journal of cell biology, Vol. 134, Issue 4, pp. 1003-18, 1996 (PubMed).

Zahraoui, Louvard, Galli: "Tight junction, a platform for trafficking and signaling protein complexes." in: The Journal of cell biology, Vol. 151, Issue 5, pp. F31-6, 2000 (PubMed).

Langbein, Grund, Kuhn et al.: "Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom." in: European journal of cell biology, Vol. 81, Issue 8, pp. 419-35, 2002 (PubMed).

Langbein, Pape, Grund et al.: "Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas." in: European journal of cell biology, Vol. 82, Issue 8, pp. 385-400, 2003 (PubMed).