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Symplekin (SYMPK) antibody

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Synonyms CG2097, Dmel\\CG2097, dSymp, sympk, MGC75595, SYMPK, ENHANCED SILENCING PHENOTYPE 4, T10O8.110, T10O8_110, T22C5.3, DKFZp468C0711, SPK, SYM, Hfn3g, 1500016F02Rik, 4632415H16Rik, AA125406, AI449890
Human, Rat (Rattus)
(40), (12), (11), (1), (1), (1)
(28), (12)
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(37), (15), (14), (11), (5), (5), (2), (1), (1), (1)
Pubmed 4 references available
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Quantity 5 mL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Human symplekin
Clone Sym-TJ-E150
Isotype IgG1
Specificity The mab reacts specifically with symplekin (a 150 kDa polypeptide) present in 1) tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis (but not their vascular endothelia!) and 2) exclusively in the nucleoplasm of these and many other cell types which do not form tight junctions (e.g. stratified epithelia such as oesophagus, tongue mucosa, epidermis, squamous cell carcinoma, nonepithelia such as fibroblasts, endothelia, lymphocytes).
Purification Supernatant
Alternative Name Symplekin / SYMPK (SYMPK Antibody Abstract)
Background Symplekin, 126.5 kD polypeptide (calculated from aa sequence data), apparent Mr 150 kD (after SDS-PAGE), pI 6.5Synonyms: SPK
Gene ID 8189
NCBI Accession NP_004810
UniProt Q92797
Application Notes Immunoblotting. Immunohistochemistry: For nucleoplasmic staining a modified Carnoy fixation procedure issuggested (60 % ethanol, 30 % chloroform, 10 % acetic acid) for 20 min, followed by a 10 min ethanol wash.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Langbein, Pape, Grund et al.: "Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas." in: European journal of cell biology, Vol. 82, Issue 8, pp. 385-400, 2003 (PubMed).

Langbein, Grund, Kuhn et al.: "Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom." in: European journal of cell biology, Vol. 81, Issue 8, pp. 419-35, 2002 (PubMed).

Zahraoui, Louvard, Galli: "Tight junction, a platform for trafficking and signaling protein complexes." in: The Journal of cell biology, Vol. 151, Issue 5, pp. F31-6, 2000 (PubMed).

Keon, Schäfer, Kuhn et al.: "Symplekin, a novel type of tight junction plaque protein." in: The Journal of cell biology, Vol. 134, Issue 4, pp. 1003-18, 1996 (PubMed).

Catalog No. ABIN112237
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