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Symplekin (SYMPK) antibody
| Antigen | Symplekin (SYMPK) |
| Synonyms | SPK, SYM, FLJ27092, DmelCG2097, AA125406, AI449890, 1500016F02Rik, 4632415H16Rik, HNF-3G, sympk, MGC75595, SYMPK, ENHANCED SILENCING PHENOTYPE 4, ESP4, T10O8.110, T10O8_110, T22C5.3, DKFZp468C0711 |
| Clonality | Monoclonal (Sym-TJ-E150) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human, Rat (Rattus) |
| Application |
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB) |
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4 references available |
| Catalog no. | ABIN112237 |
| Quantity | 5 mL |
| Price | 385.00 $ Plus shipping costs $45.00 |
| Shipping to |
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| Availability | Will be delivered in 6 to 8 Business Days |
Additional Information
| Alternative name | Symplekin / SYMPK |
| UniProt | Q92797 |
| Immunogen | Human symplekin |
| Cross-Reactivity | Human, Rat (Rattus) |
| Isotype | IgG1 |
| Clone | Sym-TJ-E150 |
| Description | Symplekin, 126. 5 kD polypeptide (calculated from aa sequence data), apparent Mr 150 kD(after SDS-PAGE), pI 6. 5 |
| Characteristics | Synonyms: SPK |
Application Details
| Application Notes | Immunoblotting. Immunohistochemistry: For nucleoplasmic staining a modified Carnoy fixation procedure issuggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10min ethanol wash. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
| Purification | Supernatant |
| Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
| Restrictions | For Research Use only |
Publications
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Keon, Schäfer, Kuhn et al.: "Symplekin, a novel type of tight junction plaque protein." in: The Journal of cell biology, Vol. 134, Issue 4, pp. 1003-18, 1996 (PubMed).
Zahraoui, Louvard, Galli: "Tight junction, a platform for trafficking and signaling protein complexes." in: The Journal of cell biology, Vol. 151, Issue 5, pp. F31-6, 2000 (PubMed). Langbein, Grund, Kuhn et al.: "Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom." in: European journal of cell biology, Vol. 81, Issue 8, pp. 419-35, 2002 (PubMed). Langbein, Pape, Grund et al.: "Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas." in: European journal of cell biology, Vol. 82, Issue 8, pp. 385-400, 2003 (PubMed). |
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Alternatives
Alternatives for antigen "Symplekin (SYMPK)", type "Antibodies"
| Hosts | Rabbit (12), Mouse (6) |
| Reactivities | Human (14), Mouse (Murine) (4), Rat (Rattus) (4), Cow (Bovine) (1) |
| Applications | Western Blotting (WB) (17), ELISA (8), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunofluorescence (IF) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (Fixed) (IHC (fx)) (1) |
| Epitopes | Internal Region (1), N-Term (1) |
