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Desmocollin 1 antibody

DSC1 Reactivity: Human WB, IHC (p), IHC (fro) Host: Mouse Monoclonal Dsc-1-U100 unconjugated
Catalog No. ABIN112238
  • Target See all Desmocollin 1 (DSC1) Antibodies
    Desmocollin 1 (DSC1)
    Reactivity
    • 37
    • 7
    • 3
    Human
    Host
    • 37
    • 2
    • 1
    Mouse
    Clonality
    • 37
    • 3
    Monoclonal
    Conjugate
    • 23
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Desmocollin 1 antibody is un-conjugated
    Application
    • 30
    • 18
    • 13
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Sequence
    HQTLESVKGV GQGDTGRYAT DWQC
    Specificity
    Reacts with 111 kDa (form "a") and 103 kDa (form "b") polypeptides (Desmocollin 1 splice forms, both at pI 5.2) from Human epidermal desmosomes. Mab localizes Dsc1 present in - suprabasal layers of interfollicular epidermis - specific cell layers (e.g. in hair follicle root sheath) - Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).
    Purification
    Affinity Chromatography on Protein A
    Immunogen
    Synthetic peptide corresponding to sequence present within the intracellular part of Human Desmocollin 1
    Clone
    Dsc-1-U100
    Isotype
    IgG1
    Top Product
    Discover our top product DSC1 Primary Antibody
  • Application Notes
    Imunoblotting. Immunohistochemistry on frozen sections: 1: 10, 1 h at RT or overnight at 2-8C,preincubation with 0.05 - 0.2 % Triton X-100, for 5-10 min, depending on tissue type, isrecommended, Without detergent pretreatment a cytoplasmic component mightcross-react (e. g. in fibroblasts). Immunohistochemistry on paraffin-embedded sections (after microwave treatment): 1: 10, 1h at RT or overnight at 2-8C, a slight crossreactivity with a lymphocyte subpopulation isabolished by mild trypsinization after the microwave step)
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in 1 mL dist. water.
    Buffer
    PBS buffer, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Desmocollin 1 (DSC1)
    Alternative Name
    Desmocollin-1 (DSC1 Products)
    Synonyms
    DSC1 antibody, CDHF1 antibody, DG2/DG3 antibody, DSC1b antibody, DG2DG3 antibody, dg3 antibody, Dsc1a antibody, Dsc1b antibody, desmocollin 1 antibody, DSC1 antibody, Dsc1 antibody
    Background
    Desmocollin 1 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It may contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Desmocollin 1 is linked to the keratinization of epithelial tissues.Synonyms: CDHF1, Cadherin family member 1, DG2/DG3, DSC1, Desmosomal glycoprotein 2/3
    Gene ID
    1823
    NCBI Accession
    NP_004939
    UniProt
    Q08554
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