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Details for Product No. ABIN112240

Desmoglein 1 (DSG1) antibody

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Antigen
Synonyms DG1, DSG, CDHF4, PPKS1, SPPK1, DSG-1, Dsg1b, Dsg1c, DSG1
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 4 references available
Catalog no. ABIN112240
Quantity 5 mL
Price
390.50 $   Plus shipping costs $45.00
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Immunogen Recombinant Human polypeptide Desmoglein 1 (extracellular part EII, EIII and EIV)
Clone Dsg1-P124
Isotype IgG2a
Specificity In Immunoblots of cytoskeletal preparations from Human skin the antibody reacts specifically with a 160 kD polypeptide typical for Desmoglein 1. In immunolocalization studies the Mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera. Cross-reaction with Desmogleins 2 and 3 was not detected.
Purification Supernatant
Alternative Name Desmoglein-1
Background Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. Desmoglein 1 has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus. Desmoglein 1 is a 165kD glycoprotein that is a core component of the desmosome cell-cell junction between epithelial cells. Desmoglein 1 is synthesized in the suprabasal layer of the skin, and is vital to epidermal integrity.
Alternate names: CDHF4, Cadherin family member 4, DG1, DGI, DSG1, Desmosomal glycoprotein 1, Pemphigus foliaceus antigen
Gene ID 1828
NCBI Accession NP_001933.2
UniProt Q02413
Research Area Cytoskeleton
Application Notes Immunohistochemistry on frozen sections: Ready-to-use, 1 h at RT, preincubation with 0.05- 0.2% Triton X-100 for 5 - 10 min, depending on tissue type, is recommended. Immunohistochemistry on paraffin-embedded tissue after MICROWAVE treatment: Ready-to-use, 1 h at RT, preincubation with 0.001% trypsin and addition of 2% milk powderto the incubation media is recommended. Immunoblotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.09% Sodium Azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Schäfer, Stumpp, Franke: "Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues." in: Differentiation; research in biological diversity, Vol. 60, Issue 2, pp. 99-108, 1996 (PubMed).

Kurzen, Moll, Moll et al.: "Compositionally different desmosomes in the various compartments of the human hair follicle." in: Differentiation; research in biological diversity, Vol. 63, Issue 5, pp. 295-304, 1998 (PubMed).

Akat, Mennel, Kremer et al.: "Molecular characterization of desmosomes in meningiomas and arachnoidal tissue." in: Acta neuropathologica, Vol. 106, Issue 4, pp. 337-47, 2003 (PubMed).

Waschke, Bruggeman, Baumgartner et al.: "Pemphigus foliaceus IgG causes dissociation of desmoglein 1-containing junctions without blocking desmoglein 1 transinteraction." in: The Journal of clinical investigation, Vol. 115, Issue 11, pp. 3157-65, 2005 (PubMed).

Alternatives for antigen "Desmoglein 1 (DSG1)", type "Antibodies"
Hosts (31), (25), (5)
Reactivities (60), (26), (18), (17), (17), (17)
Applications (38), (15), (15), (15), (15), (10), (10), (7), (7), (7), (1), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (10), (2), (2), (1), (1), (1), (1), (1), (1)
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