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26S Proteasome (p52 subunit) antibody

Antigen

26S Proteasome (p52 subunit)

Clonality Monoclonal (P52(26S-17))
Host

Mouse

Reactivity

Human, Rat (Rattus), Xenopus laevis

Application
Western Blotting (WB)
2 references available
Catalog no. ABIN112251
Quantity 5 ml
Price 265.00 $   Plus shipping costs $35.00
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Additional Information

Characteristics Synonyms: Proteasome26S
Immunogen 26S proteasomes purified from Xenopus laevis ovary.
Format Supernatant
Isotype IgG1  (Matching secondary antibodies)
Clone P52(26S-17)
Specificity The p52 subunit (Mr 52. 000) is the major antigen of the 19S cap complex, which is asubcomplex of the 26S proteasome, implicated in ATP- and ubiquitin-dependent proteindegradation. Species: Xenopus laevis. Others not tested.

Application Details

Application Notes Immunoblotting: 1: 5 to 1: 10 when using the ECL method. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Buffer 0.15 M Phosphate buffered saline with 0.1% BSA and 0.09% Na-AzideECL: 5% milk powder in TBST
Storage Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Restrictions For Research Use only

Publications

Publications Peters: "Proteasomes: protein degradation machines of the cell." in: Trends in biochemical sciences, Vol. 19, Issue 9, pp. 377-82, 1995 (PubMed).

Peters, Franke, Kleinschmidt: "Distinct 19 S and 20 S subcomplexes of the 26 S proteasome and their distribution in the nucleus and the cytoplasm." in: The Journal of biological chemistry, Vol. 269, Issue 10, pp. 7709-18, 1994 (PubMed).