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26S Proteasome antibody

Details for Product No. ABIN112251
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Antigen
Reactivity
Human, Rat (Rattus), Xenopus laevis
(4), (2), (1)
Host
Mouse
(5)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(5), (4), (2), (2)
Pubmed 2 references available
Quantity 5 mL
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Catalog No. ABIN112251
308.00 $
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Immunogen 26S proteasomes purified from Xenopus laevis ovary.
Clone P52(26S-17)
Isotype IgG1
Specificity The p52 subunit (Mr 52.000) is the major antigen of the 19S cap complex, which is a subcomplex of the 26S proteasome, implicated in ATP- and ubiquitin-dependent protein degradation.
Purification Supernatant
Background Alternate names: Proteasome26S
Application Notes Immunoblotting: 1: 5 to 1: 10 when using the ECL method. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.15 M Phosphate buffered saline with 0.1% BSA and 0.09% Na-AzideECL: 5% milk powder in TBST
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Peters, Franke, Kleinschmidt: "Distinct 19 S and 20 S subcomplexes of the 26 S proteasome and their distribution in the nucleus and the cytoplasm." in: The Journal of biological chemistry, Vol. 269, Issue 10, pp. 7709-18, 1994 (PubMed).

General Peters: "Proteasomes: protein degradation machines of the cell." in: Trends in biochemical sciences, Vol. 19, Issue 9, pp. 377-82, 1995 (PubMed).

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