Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Keratin 3 antibody

KRT3 Reactivity: Human, Rabbit, Cow WB, IHC (fro) Host: Mouse Monoclonal AE5 unconjugated
Catalog No. ABIN112318
  • Target See all Keratin 3 (KRT3) Antibodies
    Keratin 3 (KRT3)
    Reactivity
    • 31
    • 4
    • 1
    • 1
    • 1
    Human, Rabbit, Cow
    Host
    • 28
    • 5
    Mouse
    Clonality
    • 28
    • 5
    Monoclonal
    Conjugate
    • 15
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 3 antibody is un-conjugated
    Application
    • 23
    • 15
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Cytokeratin 3 antibody, clone AE5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells. Polypeptide Reacting: Mr 64 000 polypeptide (Keratin K3, formerly also designated Cytokeratin 3) of human corneal epithelium and Keratin K76 (formerly also designated Cytokeratin K2p) of palate epithelium. Reactivities on Cultured Cell Lines: Rabbit corneal epithelial cells. In Cow and Rabbit it reacts with lip and snout epithelia.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Rabbit and Bovine.
    Purification
    Ion Exchange Chromatography.
    Immunogen
    Human epithelial keratin
    Clone
    AE5
    Isotype
    IgG1
    Top Product
    Discover our top product KRT3 Primary Antibody
  • Application Notes
    Immunoblotting: 1/500. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7,2 containing 0.09 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Keratin 3 (KRT3)
    Alternative Name
    Cytokeratin 3 (KRT3 Products)
    Synonyms
    CK3 antibody, K3 antibody, CK-3 antibody, keratin 3 antibody, KRT3 antibody
    Background
    Cytokeratin 3 belongs to the intermediate filament family. It is a type II cytokeratin and is specifically expressed in the corneal epithelium. Cytokeratin 3 associates with Cytokeratin 12. Defects in Cytokeratin 3 are a cause of Meesmann corneal dystrophy.Synonyms: 65 kDa cytokeratin, KRT3, Keratin, Type-II keratin Kb3, keratin-3, keratin-76, type II cytoskeletal 3
    Gene ID
    3850
    NCBI Accession
    NP_476429
    UniProt
    P12035
You are here:
Support