CDH6 antibody (Extracellular Domain)

Catalog No. ABIN112365

Product Details anti-CDH6 Antibody

Target: CDH6 (K-Cadherin (CDH6))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDH6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Clone 2B6 recognizes the extracellular domain of Cadherin-6.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Purification: Purified

Immunogen: Affinity purified extracellular domain of human cadherin-6-GST fusion protein

Clone: 2B6

Isotype: IgG1

Application Details

Application Notes: Immunoblotting (1: 25 - 1: 250). Immunocytochemistry. Immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mMCaCl2 and 0.1 mM MgCl2. Recommended range is 1: 25 - 1: 50 for immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDH6

Target: CDH6 (K-Cadherin (CDH6))

Alternative Name: Cadherin-6 (CDH6 Products)

Synonyms: KCAD antibody, K-cadherin antibody, cad6 antibody, cadherin-6 antibody, CAD6 antibody, c-cad6B antibody, cadherin 6 antibody, Cdh6 antibody, CDH6 antibody

Background: Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. Cadherin-6 is expressed in the proximal tubule epithelium of the kidney from which most renal cell carcinomas are derived.Synonyms: CDH6, Cadherin K, K-Cadherin, Kidney cadherin

Gene ID: 1004

NCBI Accession: NP_004923

UniProt: P55285

Pathways: Cell-Cell Junction Organization