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Reticulon 1 antibody

RTN1 Reactivity: Human, Mouse, Rat, Hamster WB, IHC (fro), IHC (p), IF Host: Mouse Monoclonal MON162 unconjugated
Catalog No. ABIN112385
  • Target See all Reticulon 1 (RTN1) Antibodies
    Reticulon 1 (RTN1)
    Reactivity
    • 33
    • 31
    • 26
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Mouse, Rat, Hamster
    Host
    • 39
    • 9
    Mouse
    Clonality
    • 39
    • 9
    Monoclonal
    Conjugate
    • 24
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Reticulon 1 antibody is un-conjugated
    Application
    • 48
    • 17
    • 13
    • 13
    • 12
    • 9
    • 9
    • 8
    • 6
    • 3
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Specificity
    MON-162 exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues.
    Purification
    Purified
    Immunogen
    Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).
    Clone
    MON162
    Isotype
    IgG1
    Top Product
    Discover our top product RTN1 Primary Antibody
  • Application Notes
    Suitable for Immunocytochemistry, Immunohistochemistry on frozen and paraffinembedded tissue, and Immunoblotting. Recommended dilutions: 1/100-1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent and 1/100-1/1000 for Immunoblotting applications.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS with 0.09 % sodium azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Do not freeze working dilutions.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store lyophilized antibody at 2-8 °C. Aliquot and store reconstituted antibody at -20 °C.
  • Target
    Reticulon 1 (RTN1)
    Alternative Name
    Reticulon 1 / RTN1 (RTN1 Products)
    Synonyms
    NSP antibody, 0710005K15Rik antibody, 4930441F12Rik antibody, Nsp antibody, R75279 antibody, Rtn1-a antibody, Rtn1-b antibody, Rtn1-c antibody, reticulon-1 antibody, RTN1 antibody, RTN1-Cw antibody, rtn1 antibody, XRTN1-A antibody, XRTN1-C.1 antibody, xrtn1 antibody, xrtn1-c antibody, RTN1.2 antibody, Rtn1-C.2 antibody, rtn1-a antibody, rtn1b antibody, xRTN1 antibody, RTN1.1 antibody, rtn1a antibody, xrtn1-c.1 antibody, rtn1l antibody, wu:fa21b09 antibody, wu:fj35h01 antibody, reticulon 1 antibody, reticulon-1 antibody, reticulon 1 S homeolog antibody, reticulon 1 L homeolog antibody, reticulon 1b antibody, RTN1 antibody, Rtn1 antibody, rtn1 antibody, Rtnl1 antibody, LOC100580843 antibody, rtn1.S antibody, rtn1.L antibody, LOC100339591 antibody, rtn1b antibody
    Background
    Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non- SCLC (cell cultures). The epitope of MON-162 is located between amino acid residues 338-422 of Reticulon-1A.Synonyms: NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1
    Gene ID
    6252
    NCBI Accession
    NP_066959
    UniProt
    Q16799
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