Cardiotin antibody

Catalog No. ABIN112392

Product Details

Target: Cardiotin

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody SR-2 reacts with Cardiotin, a mitochondrionassociated protein, which is present in cardiomyocytes and skeletal muscle. SR-2 reacts with cardiomyocytes, skeletal muscle, stromal and epithelial cells as well in vivo as in vitro. In immunoblotting assays SR-2 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits.

Cross-Reactivity (Details): Species reactivity (tested):Human, Swine.

Purification: Purified

Immunogen: 100 kDa cardiotin subunit

Clone: SR-2

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry on frozen and paraffin-embedded tissue (1: 25 - 1: 100, withavidinbiotinylated horseradish peroxidase complex (ABC) as detection reagent). Immunoblotting (1: 50 - 1: 500, reacts with the 300 kDa cardiotin protein complex and its100 kDa and 60 kDa subunits).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Cardiotin

Background: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.