The antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.
The antibody is designed for quantitative determination of soluble beta2-Microglobulin/MHC Class I complexes. This antibody can also be used for detection offree beta 2-Microglobulin by Western blotting. ELISA. Western blot (Both Reducing and non-Reducing conditions): Recommended Dilution: 2-4 μg/mL. Positive Control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphomacell line. Negative Control: EL4 mouse lymphoblastic lymphoma cell line. Sample Preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing/reducing Laemmli SDS-PAGE sample buffer. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
This product is photosensitive and should be protected from light
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Poláková, Železníková, Russ: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, (2013) (PubMed).
B2M non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin