beta-2 Microglobulin antibody (beta-2-Microglobulin)

Details for Product anti-B2M Antibody No. ABIN112401, Supplier: Log in to see
Antigen
  • B2M
  • b2m
  • B2MG
  • Ly-m11
  • beta2-m
  • beta2m
  • b2m-W01
  • b2m-W03
  • b2m-Z01
  • b2m-Z02
  • b2m-Z03
  • bwm
  • wu:fa94c05
  • wu:fb10a09
  • beta-2-microglobulin
  • beta-2 microglobulin
  • Beta-2-microglobulin-like
  • B2M
  • b2m
  • B2m
  • LOC100350679
Alternatives
anti-Human beta-2 Microglobulin antibody for Affinity Purification
Reactivity
Human
626
86
78
49
19
19
14
12
9
7
7
3
2
2
2
2
1
1
1
1
1
Host
Mouse
495
171
35
25
12
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
This beta-2 Microglobulin antibody is un-conjugated
52
42
33
33
16
15
15
14
14
12
11
11
11
11
11
10
8
8
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5
5
3
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
405
339
298
196
141
114
73
51
44
38
29
13
12
12
10
9
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Immunogen Purified Human beta-2-Microglobulin
Clone B2M-01
Isotype IgG2a
Specificity The antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein A
Alternative Name beta-2-Microglobulin (B2M Antibody Abstract)
Background Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin
Gene ID 567
NCBI Accession NP_004039
UniProt P61769
Research Area Serum/Plasma Proteins, Coagulation
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Application Notes Flow CytometryDilution: 1 mg/mLPositive control: PBL cell line,Negative control: DAUDI human lymphoma cell line, erythrocytes. ImmunoprecipitationB2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from thelysates of human lymphoid cells. RIAThe dissociation constant of the antibody soluble beta2-Microglobulin is 1.5 x 10e-8 Mol/las determined by competitive RIA. ELISA. Immunohistochemistry (Paraffin)Dilution: 10 μg/mL. Positive tissue: kidney glomeruli. Western blotting (Reducing and Non-Reducing Conditions). Dilution: 2-4 μg/mL. Positive control: U937 human histiocytic ymphoma cell line. Negative control: EL4 mouse lymphoblastic lymphoma cell line. Sample Preparation (See Protocols).
Protocol Sample Preparation (Western Blotting): Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mMTris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Protease inhibitor Cocktail). Incubate60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducingLaemmli SDS-PAGE sample buffer.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Khurana, Traum, Aivado, Wells, Guerrero, Grall, Libermann, Schachter: "Urine proteomic profiling of pediatric nephrotic syndrome." in: Pediatric nephrology (Berlin, Germany), Vol. 21, Issue 9, pp. 1257-65, 2006 (PubMed).

Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, 1985 (PubMed).