M13 Bacteriophage G8P Capsid antibody

Antigen: M13 Bacteriophage G8P Capsid

Clonality: Monoclonal (RL-ph3)

Host: Mouse

Application: Western Blotting (WB)

Catalog no.: ABIN112404

Additional Information

Characteristics: Synonyms: Gene 8 protein, Coat protein B, Major coat protein

Swiss-Prot: P69541

Immunogen: Isolated M13 phage coat proteins. Remarks: RL-ph3 is a mouse monoclonal IgM, k antibody derived by fusion of SP2/0-Ag14mouse myeloma cells with spleen cells from a BALB/c mouse immunized with isolated M13phage coat proteins.

Isotype: IgM  (Matching secondary antibodies)

Clone: RL-ph3

Description: The display of repertoires of antibody fragments on the surface of filamentous phage offersa new way to produce immunoreagents with defined specificities. Phage derived antibodyfragments offer a number of advantages over mouse monoclonal antibodies, such asbetter clearance from the blood, the possibility to select from human combinatoriallibraries and the relative ease by which such fragments can be manipulated. The phagedisplay technique thus facilitates the selection of antibody fragments of therapeutic valueor research interest. Antibodies to M13 filamentous phage coat proteins are instrumental inthe selection and detection of phages expressing specific antibody fragments or peptidesequences at their surface.

Specificity: RL-ph3 reacts with a M13 filamentous phage coat protein with a molecular weight ofapproximately 5 kDa.

Application Details

Application Notes: RL-ph3 is paticularly useful for detection of M13 phages in multistep Immunoblottingprocedures (1/40-1/400). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS with 0.09% Sodium Azide as preservative.

Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Restrictions: For Research Use only

Publications

Publications: Meulemans, Nieland, Debie et al.: "Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody." in: Human antibodies and hybridomas, Vol. 6, Issue 3, pp. 113-8, 1996 (PubMed).