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Nestin antibody

NES Reactivity: Mouse, Rat WB, IF, IHC (p), IHC (fro) Host: Mouse Monoclonal 4D4 (Rat-401) unconjugated
Catalog No. ABIN112422
  • Target See all Nestin (NES) Antibodies
    Nestin (NES)
    Reactivity
    • 129
    • 67
    • 46
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse, Rat
    Host
    • 103
    • 33
    • 4
    • 2
    • 1
    Mouse
    Clonality
    • 108
    • 35
    Monoclonal
    Conjugate
    • 61
    • 10
    • 10
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    This Nestin antibody is un-conjugated
    Application
    • 78
    • 50
    • 48
    • 47
    • 40
    • 39
    • 38
    • 29
    • 24
    • 14
    • 7
    • 6
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody recognizes Mouse and Rat Nestin and does not recognizes Human Nestin.
    Purification
    Ig Fraction
    Immunogen
    Nestin purified from embryonic rat spinal cord. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
    Clone
    4D4 (Rat-401)
    Isotype
    IgG
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    Discover our top product NES Primary Antibody
  • Application Notes
    Western Blot: reacts with a band at 200-220 kDa in reducing gels of newborn rat or mousecell extracts, For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol and4.6 % SDS, Membranes should be blocked with milk or BSA, 5 % PAGE gels are suggested. Immunohistochemistry on frozen and paraffin sections: 1/20 - 1/200, We recommendperfusing tissues with 4 % paraformaldehyde at pH 7.4 for light microscopy or with either4 % paraformaldehyde at pH 10.0 or 4 % paraformaldehyde with 0.1 % glutaraldehyde at pH 7.4 for EM, For Immunocytochemistry we recommend using cells fixed in 4 %paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    TRIS buffered saline, 0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Nestin (NES)
    Alternative Name
    Nestin (NES Products)
    Synonyms
    paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody
    Background
    Nestin is a large intermediate filament protein originally identified by Hockfield and McKay (1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.Synonyms: NES, Nbla00170
    Gene ID
    18008
    NCBI Accession
    NP_057910
    UniProt
    Q6P5H2
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