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Antibody
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anti-Gap Junction Protein alpha 1 43kDa GJA1
Gap Junction Protein, alpha 1, 43kDa (GJA1) antibody
| Antigen |
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| Synonyms |
CX43, GJAL, ODDD, DFNB38, MGC128721, gja1-A, connexin 43, cx43, gjal, oddd, dfnb38, connexin43, Cx43 |
| Clonality |
Monoclonal (4E6-2) |
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Host
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Reactivity
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Human (30), Mouse (Murine) (23), Rat (Rattus) (22), Dog (Canine) (8), Chicken (6), Zebrafish (Danio rerio) (6), Cow (Bovine) (3), Guinea Pig (3), Sheep (Ovine) (3), Pig (Porcine) (2), Xenopus laevis (2), Bird (Avian) (1), Finch (1), Frog (1), Monkey (1), Primate (1), Rabbit (1)
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
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| Catalog no. |
ABIN112539 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
990.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Gap junction alpha-1 protein, Connexin-43, GJAL, Gap junction 43 kDa heart protein
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Alternative name
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GJA1 / Cx43
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Swiss-Prot
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P17302
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Immunogen
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Synthetic peptide corresponding to amino acids 252-270 of the mouse Connexin 43sequence
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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4E6-2
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Description
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GJA1 is a member of the connexin gene family and a component of gap junctions. Gapjunctions are composed of arrays of intercellular channels and provide a route for thediffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein ofgap junctions in the heart, and gap junctions are thought to have a crucial role in thesynchronized contraction of the heart and in embryonic development. GJA1 is targeted byseveral protein kinases that regulate myocardial cell cell coupling. A related intronlessGJA1 pseudogene, GJA1P, has been mapped to chromosome 5.
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Specificity
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The antibody recognizes Connexin 43. Species: Mouse, rat, dog, bovine, pig, human. Others not tested.
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Application Notes
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Western blotting (1: 1,000 - 1: 10,000): the antibody recognises proteins of 43-47 kD onWestern blots of mouse brain lysate. ELISA. Immunofluorescence. Immunohistochemistry (1/50 - 1/250 on frozen tissues). Can be used for the detection ofconnexin 43 in mouse keratinocyte cells and on frozen sections of rat heart. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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PBS, pH 7.2, containing 0.09% sodium azide
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Gap Junction Protein, alpha 1, 43kDa (GJA1)", type "Antibodies"
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Hosts
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Rabbit (29), Goat (2), Mouse (1)
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Reactivities
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Human (30), Mouse (Murine) (23), Rat (Rattus) (22), Dog (Canine) (8), Chicken (6), Zebrafish (Danio rerio) (6), Cow (Bovine) (3), Guinea Pig (3), Sheep (Ovine) (3), Pig (Porcine) (2), Xenopus laevis (2), Bird (Avian) (1), Finch (1), Frog (1), Monkey (1), Primate (1), Rabbit (1)
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Applications
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Western Blotting (WB) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunofluorescence (IF) (1), Immunoprecipitation (IP) (1)
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Epitopes
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pSer368 (7), C-Term (2), pSer367 (2), N-Term (1)
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