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Antibodies
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anti-Choline O-Acetyltransferase (CHAT) antibody
Choline O-Acetyltransferase (CHAT) antibody
| Antigen |
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| Synonyms |
CMS1A, CMS1A2, B230380D24Rik, CT23399, Cat, ChAT, cha, dChAT, l(3)91Cc, DmelCG12345, CG12345, CHAT, ChoAcTase, Ci-ChAT |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Cat (Feline), Human, Monkey, Mouse (Murine), Rat (Rattus)
Human (45), Rat (Rattus) (38), Mouse (Murine) (35), Pig (Porcine) (20), Dog (Canine) (19), Guinea Pig (11), Rabbit (5), Chicken (4), Bird (Avian) (2), Cow (Bovine) (2), Horse (Equine) (2), Primates (2), Monkey (1)
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Application
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Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
Western Blotting (WB) (38), Immunohistochemistry (IHC) (26), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Enzyme Immunoassay (EIA) (4), Immunocytochemistry (ICC) (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
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| Catalog no. |
ABIN112862 |
| Quantity |
1 ml (5.0 mg/ml) (Variants) |
| Price |
330.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Choline O-acetyltransferase, Choline acetylase, CHOACTase, ChAT, EC=2.3.1.6
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Alternative name
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CHAT
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Swiss-Prot
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P28329
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Immunogen
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Native Choline Acetyltransferase from Human placenta.
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Description
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Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitteracetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as aspecific marker for cholinergic neurons in both peripheral and central nervous systems. Dysfunction of cholinergic neurons underlies aspects of clinical symptoms found inneurological and psychiatric disorders such as Alzheimer's disease, Down and Rettsyndromes. Choline acetyltransferase recruits choline for the synthesis of acetylcholine atpreganglionic parasympathetic and sympathetic fibres. It is also present atparasympathetic postganglionic fibres.
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Specificity
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Cholinergic neurons in brain and central nervous system. Species: Human, Rat, Mouse, Cat, Monkey and Bat. Others not tested.
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Application Notes
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ELISA (1/250-1/1000): In a simple ELISA with 1 µg of antigen coated per well, the 50% ODwas observed at an antibody dilution of 1: 500. Under the same conditions the end pointwas observed at 1/10,000). Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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5.0 mg/ml
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Buffer
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PBS containing 0.09% Sodium Azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Choline O-Acetyltransferase (CHAT)", type "Antibodies"
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Hosts
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Rabbit (41), Goat (15), Sheep (7), Mouse (6), Chicken (5)
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Reactivities
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Human (45), Rat (Rattus) (38), Mouse (Murine) (35), Pig (Porcine) (20), Dog (Canine) (19), Guinea Pig (11), Rabbit (5), Chicken (4), Bird (Avian) (2), Cow (Bovine) (2), Horse (Equine) (2), Primates (2), Monkey (1)
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Applications
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Western Blotting (WB) (38), Immunohistochemistry (IHC) (26), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Enzyme Immunoassay (EIA) (4), Immunocytochemistry (ICC) (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (2), internal (2), N-Term (1)
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