Coagulation Factor V (F5) antibody

Antigen: Coagulation Factor V (F5)

Synonyms: FVL, PCCF, Cf5, Cf-5, AI173222, F5

Clonality: Polyclonal

Host: Horse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN113026

Additional Information

Characteristics: Synonyms: Proaccelerin labile factor, Activated protein C cofactor

Swiss-Prot: P12259

Immunogen: Purified Human Thrombin (derived from purified prothrombin).

Description: Coagulation factor V is a cofactor that participates with factor Xa to activate prothrombin tothrombin. Factor Va is composed of a heavy chain and a light chain, noncovalently bound. The interaction between the two chains is calcium-dependent. Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of aheavy chain at the N-terminus and a light chain at the C-terminus). Sulfation is required forefficient thrombin cleavage and activation and for full procoagulant activity. Defects in F5are the cause of Owren parahemophilia which is an hemorrhagic diastesis. Defects in F5 are the cause of resistance to activated protein C (APCR). APCR is a form ofthrombophilia. The APCR mutation is found in about 5% of the population which suggestthat a slight thrombotic tendency may confer some advantage in fetal implantation.

Specificity: Factor V, Factor Va and their activation products. Species: Human. Others not tested.

Application Details

Application Notes: Suitable for use in ELISA and Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10 mg/ml (OD280nm, E1% = 14)

Buffer: 50% (vol/vol) glycerol/water without preservative.

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only