Amyloid beta (Abeta) (AA 32-40) antibody

Antigen: Amyloid beta (Abeta)

Synonyms: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, CTFgamma, aaa, ad1, pn2, abpp, appi, cvap, abeta, MGC88882, ctfgamma, APP

Binding Site: AA 32-40

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA

Catalog no.: ABIN113076

Additional Information

Alternative name: Amyloid beta

Immunogen: Peptide (amino acids 32-40) conjugated to keyhole limpet hemocyanin (KLH).

Cross-Reactivity: Human

Description: Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor andtransmembrane precursor protein that is cleaved by secretases to form a number ofpeptides. Multiple transcript variants encoding several different isoforms have been foundfor this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the mostabundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with thehighest levels in brain, kidney, heart and spleen with weak expression observed in liver,ABPP is induced during neuronal differentiation. In the adult brain, highest expression ofABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortexopercular gyri, moderate expression in the cerebellar cortex, the posterior perisylviancortex opercular gyri and the temporal associated cortex. Weak expression is found in thestriate, extra striate and motor cortices. Mutations in ABPP have been implicated inautosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloidangiopathy).

Specificity: Specific for the C-terminus of Amyloid beta 1-40. Reacts extremely well with the 1-40isoform. Does not cross-react with Amyloid beta 1-42 or Amyloid Precursor Protein (APP). Species: Human. Others not tested.

Application Details

Application Notes: ELISAWestern blotImmunohistochemistry: Epitope must be re-exposed in fixed tissue by pretreatment oftissue using one of the following procedures: 1. Formic acid (70 %) for 10-30 minutes at room temperature.2. HIER (heat Induced Epitope Retrieval) with buffers. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing no preservatives.

Storage: Store undiluted in -20°C to -70°C. Do not store antibody diluted below 50 ug/ml in theabsence of protein (i. e. add 2 % BSA). Avoid repeated freezing and thawing. Should thisproduct contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.

Research Area: Neurology, Alzheimer's Disease

Restrictions: For Research Use only

Publications

Potempska, Mack, Mehta et al.: "Quantification of sub-femtomole amounts of Alzheimer amyloid beta peptides." in: Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis, Vol. 6, Issue 1, pp. 14-21, 1999 (PubMed).

Mayeux, Tang, Jacobs et al.: "Plasma amyloid beta-peptide 1-42 and incipient Alzheimer's disease." in: Annals of neurology, Vol. 46, Issue 3, pp. 412-6, 1999 (PubMed).

Emmerling, Morganti-Kossmann, Kossmann et al.: "Traumatic brain injury elevates the Alzheimer's amyloid peptide A beta 42 in human CSF. A possible role for nerve cell injury." in: Annals of the New York Academy of Sciences, Vol. 903, pp. 118-22, 2000 (PubMed).

Tapiola, Pirttilae, Mehta et al.: "Relationship between apoE genotype and CSF beta-amyloid (1-42) and tau in patients with probable and definite Alzheimer's disease." in: Neurobiology of aging, Vol. 21, Issue 5, pp. 735-40, 2000 (PubMed).

Schupf, Patel, Silverman et al.: "Elevated plasma amyloid beta-peptide 1-42 and onset of dementia in adults with Down syndrome." in: Neuroscience letters, Vol. 301, Issue 3, pp. 199-203, 2001 (PubMed).