Chemokine (C-X-C Motif) Receptor 4 (CXCR4) antibody

Antigen: Chemokine (C-X-C Motif) Receptor 4 (CXCR4)

Synonyms: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E, Sdf1r, Cmkar4, PB-CKR, PBSF/SDF-1, MGC108696, CXCR4, MGC128682, cxcr4, cxcr4-B, CXC-R4-B, CXCR-4-B, MGC52882, MGC130802, fb22, hm89, lap3, lcr1, npyr, whim, cd184, lestr, npy3r, npyrl, CXC-R4, CXCR-4, hsy3rr, npyy3r, xcxcr4, d2s201e

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN113102

Additional Information

Alternative name: CD184 / CXCR4

UniProt: P61073

Immunogen: Peptide corresponding to amino acids 182-196 in the second extracellular loop (EL) ofHuman CXCR4

Cross-Reactivity: Human, Mouse (Murine)

Description: CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T cell tropic strains of HIV1fusion and entry of human white blood cells. CXCR4 is also required for the infection bydual tropic strains of HIV1 and mediates CD4 independent infection by HIV2. The achemokine SDF1 is the ligand for CXCR4 and prevents infection by T tropic HIV1. CXCR4associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4messenger RNA levels correlated with HIV1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV1 and HIV2 fusion and infection of human target cells. Theamino terminal domain and the second extracellular loop of CXCR4 serve as HIV bidingsites. CXCR4 is highly expressed in brain and heart, and in white blood cells, vascularendothelial cells, and umbilical cord endothelial cells.

Characteristics: Synonyms: CXC-R4, CXCR-4, Fusin, LCR1, FB22, NPYRL, HM89, SDF1 receptor, LESTR, C-X-C chemokinereceptor type 4, Stromal cell-derived factor 1 receptor, Leukocyte-derived seventransmembrane domain receptor

Synonyms: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E, Sdf1r, Cmkar4, PB-CKR, PBSF/SDF-1, MGC108696, CXCR4, MGC128682, cxcr4, cxcr4-B, CXC-R4-B, CXCR-4-B, MGC52882, MGC130802, fb22, hm89, lap3, lcr1, npyr, whim, cd184, lestr, npy3r, npyrl, CXC-R4, CXCR-4, hsy3rr, npyy3r, xcxcr4, d2s201e

Application Details

Application Notes: Western blot (1/500-1/1,000). Immunoprecipitation. Immunocytochemistry. Positive Control: HeLa whole cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Affinity Purified

Buffer: PBS with 0.02% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells, Chemokines, Receptors

Restrictions: For Research Use only

Publications

Feng, Broder, Kennedy et al.: "HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor." in: Science (New York, N.Y.), Vol. 272, Issue 5263, pp. 872-7, 1996 (PubMed).

Doranz, Rucker, Yi et al.: "A dual-tropic primary HIV-1 isolate that uses fusin and the beta-chemokine receptors CKR-5, CKR-3, and CKR-2b as fusion cofactors." in: Cell, Vol. 85, Issue 7, pp. 1149-58, 1996 (PubMed).

Berson, Long, Doranz et al.: "A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus type 1 strains." in: Journal of virology, Vol. 70, Issue 9, pp. 6288-95, 1996 (PubMed).

Bleul, Farzan, Choe et al.: "The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry." in: Nature, Vol. 382, Issue 6594, pp. 829-33, 1997 (PubMed).

Oberlin, Amara, Bachelerie et al.: "The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1." in: Nature, Vol. 382, Issue 6594, pp. 833-5, 1997 (PubMed).

Lapham, Ouyang, Chandrasekhar et al.: "Evidence for cell-surface association between fusin and the CD4-gp120 complex in human cell lines." in: Science (New York, N.Y.), Vol. 274, Issue 5287, pp. 602-5, 1996 (PubMed).

Endres, Clapham, Marsh et al.: "CD4-independent infection by HIV-2 is mediated by fusin/CXCR4." in: Cell, Vol. 87, Issue 4, pp. 745-56, 1997 (PubMed).

Brelot, Heveker, Pleskoff et al.: "Role of the first and third extracellular domains of CXCR-4 in human immunodeficiency virus coreceptor activity." in: Journal of virology, Vol. 71, Issue 6, pp. 4744-51, 1997 (PubMed).

Dimitrov: "How do viruses enter cells? The HIV coreceptors teach us a lesson of complexity." in: Cell, Vol. 91, Issue 6, pp. 721-30, 1998 (PubMed).