ACE2 (N-Term) antibody

Antigen: ACE2

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN113167

Additional Information

Characteristics: Synonyms: Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-convertingenzyme homolog

Alternative name: ACE2 (N-term)

Swiss-Prot: Q9BYF1

Immunogen: Synthetic peptide corresponding to amino acids 18 to 31 of human ACE2 (Genbankaccession NP_068576)

Description: Angiotensin converting enzyme 2 (ACE2) plays a central role in vascular, renal, andmyocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted toheart, kidney, and testis. Recently ACE2 has also been shown to be a functional receptor ofthe SARS coronavirus. The normal function of ACE2 is to convert the inactivevasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1 7, unlikeACE, which converts AngI to AngII. While the role of these vasoactive peptides is not wellunderstood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiaccontractility, indicating its importance in regulating heart function. ACE1 inhibitors such ascatopril and lisinopril do not block ACE2 activity. The full length sequence for ACE2encodes an 805 amino acid protein with predicted mass of 92. 46 kDa and a pI of 5. 22.

Specificity: Reacts with Human Angiotensin Converting Enzyme 2 (ACE2, SARS Receptor) N-terminal,amino acids 18-31. Does not cross react with ACE1. Species Reactivity: Tested: Human.

Application Details

Application Notes: Suitable for use in Western blot (0.5-2 µg/ml). Human kidney or Human testis cell lysate can be used as Positive Control and a 90 kDaband can be detected. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 0.02% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8 °C. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.

Research Area: Metabolism, Enzymes, Serum/Plasma Proteins

Restrictions: For Research Use only

Publications

Publications: Tipnis, Hooper, Hyde et al.: "A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33238-43, 2000 (PubMed).

Crackower, Sarao, Oudit et al.: "Angiotensin-converting enzyme 2 is an essential regulator of heart function." in: Nature, Vol. 417, Issue 6891, pp. 822-8, 2002 (PubMed).

Li, Moore, Vasilieva et al.: "Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus." in: Nature, Vol. 426, Issue 6965, pp. 450-4, 2003 (PubMed).