alpha-2-HS-Glycoprotein (AHSG) antibody

Antigen: alpha-2-HS-Glycoprotein (AHSG)

Synonyms: AHS, A2HS, HSGA, FETUA, fetuin-A, pp63, Aa2-066, AHSG, ahs, a2hs, hsga, fetua, MGC103687, MGC192819, wu:fb63g02, zgc:103687, MGC116429, Ahsg

Clonality: Polyclonal

Host: Sheep

Reactivity: Human

Application: Immunodiffusion (ID)

Catalog no.: ABIN113170

Additional Information

Characteristics: Synonyms: Fetuin A, AHSG, FETUA, PRO2743, Ba-alpha-2-glycoprotein, Alpha-2-Z-globulin

Alternative name: Alpha-2-HS-glycoprotein

Swiss-Prot: P02765

Immunogen: Human alpha-2-HS-Glycoprotein, purified from human serum. >95% pure (SDS-PAGE).

Description: Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized byhepatocytes. The AHSG molecule consists of two polypeptide chains, which are bothcleaved from a proprotein encoded from a single mRNA. It is involved in several functions,such as endocytosis, brain development and the formation of bone tissue. The protein iscommonly present in the cortical plate of the immature cerebral cortex and bone marrowhemopoietic matrix, and it has therefore been postulated that it participates in thedevelopment of the tissues. However, its exact significance is still obscure. It promotesendocytosis, possesses opsonic properties and influences the mineral phase of bone. Itshows affinity for calcium and barium ions.

Specificity: Gives a single arc when tested by IEP and 2D-IEP against human serum. Identity has beenconfirmed by double diffusion (Ouchterlony) against human serum and a knownanti-human alpha-2-HS glycoprotein. Species: Human. Does not cross react with: Bovine, Cat, Chicken, Dog, Goat, Rabbit,Guinea Pig, Horse, Mouse, Pig and Rat. The cross-reactivity of this product against the sera

Application Details

Application Notes: Suitable for use in a variety of gel techniques including radial immunodiffusion (RID),double diffusion, and immunoelectrophoresis (IEP). The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. RID: 5 l antiserum/cm2 in gel vs 5 l serum, NT - 1: 10. Double Diffusion: 10 l antiserum vs 2 l serumIEP: 100 l antiserum vs 5 l serumOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 19 mg/ml (OD280nm, E1% = 14.5)

Buffer: Glycine buffered saline, pH 7.4, containing 0.09% Sodium Azide, 0.1%E-Amino-n-Caproic Acid, 0.01% Benzamidine, and 1mM EDTA

Storage: Store the antibody undiluted at 2-8 °C. Slight precipitation can occur on storage, whichmay be removed by centrifugation, and should not affect performance characteristics. Shelf life: one year from despatch.

Research Area: Immunology, Inflammation, Serum/Plasma Proteins

Restrictions: For Research Use only