S100A8/A9 Complex (Calprotectin) antibody (HRP)

Catalog No. ABIN113343

Product Details anti-S100A8/A9 Complex (Calprotectin) Antibody (HRP)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))

Reactivity: Human, Cow, Pig

Host: Chicken

Clonality: Polyclonal

Conjugate: This S100A8/A9 Complex (Calprotectin) antibody is conjugated to HRP

Application: Enzyme Immunoassay (EIA)

Specificity: The antibody does not discriminate between the individual subunits MRP8 or MRP14. In immunohistochemistry, it detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears. The MRP8/14 specific ELISA has shown significant advantages in the early assessment of acute inflammatory conditions like certain bowel diseases or transplant rejection. The antibody reacts with a subpopulation of macrophages, monocytes and granulocytes, peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Antigen Distribution Isolated Cells: A subpopulation of monocytes and neutrophilic granulocytes are positive. Up to 80 % of monocytes in early cultures (24-48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue Sections: Macrophages in the red pulp of the spleen and in the liver, strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen, absent on normal resident mononuclear phagocytes in healthy tissues (skin, gut, thymus).Antigen Distribution

Purification: Repeated Precipitation

Immunogen: Recombinant MRP8/14. The antigen is produced by the Ca2+-dependent heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.

Application Details

Application Notes: Immunohistochemistry. ELISA (1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris/HCl, pH 7.2, 0.09 % Kathon, 0.1 % Phenol, 0.5 % BSA

Preservative: Kathon CG

Precaution of Use: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))

Alternative Name: MRP8 / MRP14 (S100A8/A9 Products)

Synonyms: 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, S100 calcium binding protein A9 antibody, S100A9 antibody

Background: MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.Synonyms: CAGA, CAGB, CFAG, Calgranulin A/B, Calprotectin, L1 Protein, MRP-14, MRP-8, P14, P8

Pathways: S100 Proteins