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Keratin 9 (KRT9) (AA 450-477) antibody

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Synonyms CK-9, EPPK, K9, Krt1-9
AA 450-477
(17), (14), (14), (8), (6), (5), (5), (2), (2), (2), (2), (1), (1), (1)
Cow (Bovine), Hamster, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(46), (1), (1), (1), (1)
Guinea Pig
(33), (10), (3)
(2), (2), (2), (2), (2), (2)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(40), (22), (18), (16), (15), (10), (6), (2), (1)
Pubmed 2 references available
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Quantity 0.1 mL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen KLH conjugated Synthetic peptide corresponding to amino acids 450-477 of Human keratin K9
Specificity The antiserum represents an excellent marker to study palmoplantar epidermal distribution and differentiation, specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. K9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). K9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum (See references 1 and 2).
Cross-Reactivity (Details) Species reactivity (tested):Human, Bovine, Mouse, Rat.
Purification Serum
Alternative Name Cytokeratin 9 (KRT9 Antibody Abstract)
Background Acidic keratin K9 (MW 62,129, formerly also designated cytokeratin 9), expressed in the palmoplantar epidermis.Synonyms: CK9, Cytokeratin 9, K9, KRT9, Keratin, Keratin-9, Spermatid Perinuclear Ring Manchette Protein K9, Type I cytoskeletal 9
Gene ID 3857
NCBI Accession NP_000217
UniProt P35527
Research Area Signaling, Extracellular Matrix
Application Notes Immunoblotting: 1/5000-1/10,000 for Western Blot (ECL). Immunohistochemistry on Frozen Sections: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50 (microwave retrieval method isrecommended). Incubation Time: 1 h at RT, extended with Paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.09 % Sodium Azide 0.9 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Swensson, Langbein, McMillan et al.: "Specialized keratin expression pattern in human ridged skin as an adaptation to high physical stress." in: The British journal of dermatology, Vol. 139, Issue 5, pp. 767-75, 1999 (PubMed).

Langbein, Heid, Moll et al.: "Molecular characterization of the body site-specific human epidermal cytokeratin 9: cDNA cloning, amino acid sequence, and tissue specificity of gene expression." in: Differentiation; research in biological diversity, Vol. 55, Issue 1, pp. 57-71, 1994 (PubMed).

Catalog No. ABIN113454
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