Keratin 15 (KRT15) (C-Term) antibody

Details for Product No. ABIN113456
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Antigen
Synonyms xlk2, CK15, K15, K1CO, AI528832, Krt1-15, Ka15, wu:fa91c06, wu:fa95d04, zgc:55334
Epitope
C-Term
(16), (6), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(101), (53), (51), (34), (29), (17), (12), (2), (2), (1)
Host
Guinea Pig
(74), (28), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(64), (34), (32), (28), (24), (20), (20), (11), (8), (3), (2), (1)
Pubmed 3 references available
Quantity 0.1 mL
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Catalog No. ABIN113456
511.50 $
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Immunogen C-terminal tail region (including parts of the rod domain) of human recombinant keratin K15
Specificity Mr 50 kD (pI 4.9) intermediate filament polypeptide, keratin K15 (formerly also designated cytokeratin 15), detected by immunofluorescence microscopy in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (e.g. broncheal epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicle, staining was restricted to the outermost cell layer of the outer root sheath, within the hair follicle several cell layers of the hair follicle bulb were positive with antiserum gp 15.1. In human fetal tissues keratin K15 was localized in basal cells of epidermis, lung, trachea, tracheal glands and esophagus. In fetal hair anlagen, all cells were positive with antiserum gp 15.1, suggesting that keratin K15 is preferentially expressed in epithelial stem cells. Completely unreactive were cells of liver, colon, kidney, urinary bladder, pancreas and lung alveolus. In tumors, gp15.1 is reactive with squamous skin and lung carcinomas and with a significant subgroup of invasive ductal breast carcinomas, especially tumors with higher grade malignancy (Heid, Bartek, Leube, Moll, Kaufmann & Franke, unpublished results).
Purification Serum
Alternative Name Cytokeratin 15
Background Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype.
Alternate names: CK-15, Cytokeratin-15, K15, KRT15, KRTB, Keratin, Keratin 15, Keratin-15, Type I Cytoskeletal 15
Gene ID 3866
NCBI Accession NP_002266
UniProt P19012
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Immunohistochemistry on frozen sections: 1/100 - 1/200. Western blot: 1/2000 (ECL). Incubation time: 1h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Jih, Lyle, Elenitsas et al.: "Cytokeratin 15 expression in trichoepitheliomas and a subset of basal cell carcinomas suggests they originate from hair follicle stem cells." in: Journal of cutaneous pathology, Vol. 26, Issue 3, pp. 113-8, 1999 (PubMed).

Boehnke, Mirancea, Pavesio et al.: "Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents." in: European journal of cell biology, Vol. 86, Issue 11-12, pp. 731-46, 2007 (PubMed).

General Heid, OFallon, Schwenk et al.: "Increasing the proportion of women in academic medicine: one institution's response." in: Mayo Clinic proceedings. Mayo Clinic, Vol. 74, Issue 2, pp. 113-9, 1999 (PubMed).

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