anti-CD3 antibody (CD3)

Details for Product anti-CD3 Antibody No. ABIN114139
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Synonyms CD3
Mouse (Murine)
(600), (159), (64), (32), (28), (20), (16), (7), (6), (6), (5), (3), (2)
(636), (97), (94), (50), (1)
Clonality (Clone)
Monoclonal ()
This CD3 antibody is conjugated to Biotin
(145), (89), (87), (38), (9), (9), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS)
(643), (175), (125), (113), (110), (91), (66), (64), (63), (27), (11), (10), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 0.3 mg
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Clone 500-A2
Isotype IgG
Specificity This anti-mouse CD3ε monoclonal antibody is specific for the ε chain of mouse CD31. When immobilized this antibody can be used to stimulate mouse T cells in vitro1. A combination of this antibody and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells.
Alternative Name CD3 (CD3 Antibody Abstract)
Background In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The ε chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transduction.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 12501
NCBI Accession NP_031674
UniProt P22646
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry (see Protocols). (Reportet to be useful in immunoprecipitation, immunofluorescent staining andimmuno-histochemistry on acetone fixed frozen sections. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1. 0 µg* of this AB per 1x10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-PE) at a 1: 20 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. MEDIAA. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Hamster IgG
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, 0.1 % NaN3 and EIA grade BSA as stabilizing protein to bring the total protein concentration to 4-5 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-CD3 (CD3) antibody (Biotin) Cell Source: Spleen Percentage of cells stained above control: 44.1%
Background publications Havran, Poenie, Kimura et al.: "Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes." in: Nature, Vol. 330, Issue 6144, pp. 170-3, 1987 (PubMed).

Havran, Grell, Duwe et al.: "Limited diversity of T-cell receptor gamma-chain expression of murine Thy-1+ dendritic epidermal cells revealed by V gamma 3-specific monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 11, pp. 4185-9, 1989 (PubMed).

Harding, McArthur, Gross et al.: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992 (PubMed).

General Havran, Allison: "Developmentally ordered appearance of thymocytes expressing different T-cell antigen receptors." in: Nature, Vol. 335, Issue 6189, pp. 443-5, 1988 (PubMed).

Validation Images
Catalog No. ABIN114139
885.50 $
Plus shipping costs $45.00
0.3 mg
885.50 $

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