CD3 antibody (CD3)

Details for Product anti-CD3 Antibody No. ABIN114142, Supplier: Log in to see
Antigen
  • CD3
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • DNA segment, Chr 9, MRC UK Mouse Genome Centre 45 expressed
  • CD3D
  • CD3E
  • D9Mgc45e
Reactivity
Mouse (Murine)
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30
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Host
Hamster
889
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62
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22
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD3 antibody is un-conjugated
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Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
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Options
Supplier
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Clone 500-A2
Isotype IgG
Specificity This anti-mouse CD3epsilon monoclonal antibody is specific for the epsilon chain of mouse CD31. Immobolized 500A2 mAb can be used to stimulate mouse T cells in vitro1. A combination of immobilized 500A2 mAb and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells3.
Purification Purified
Alternative Name CD3 (CD3 Antibody Abstract)
Background In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The epsilon chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transduction.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 12501
NCBI Accession NP_031674
UniProt P22646
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry (see Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer PBS, 0.09 % NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Harding, McArthur, Gross, Raulet, Allison: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992 (PubMed).

Havran, Grell, Duwe, Kimura, Wilson, Kruisbeek, OBrien, Born, Tigelaar, Allison: "Limited diversity of T-cell receptor gamma-chain expression of murine Thy-1+ dendritic epidermal cells revealed by V gamma 3-specific monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 11, pp. 4185-9, 1989 (PubMed).

Havran, Allison: "Developmentally ordered appearance of thymocytes expressing different T-cell antigen receptors." in: Nature, Vol. 335, Issue 6189, pp. 443-5, 1988 (PubMed).

Havran, Poenie, Kimura, Tsien, Weiss, Allison: "Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes." in: Nature, Vol. 330, Issue 6144, pp. 170-3, 1987 (PubMed).

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