CD8 antibody (PE)

Catalog No. ABIN114168

Product Details anti-CD8 Antibody (PE)

Target: CD8

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This anti-CD8a (Ly 2) monoclonal antibody reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (ie. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype.). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology. (1) It can also be useful to identify/eliminate cytotoxic or suppressor T lymphocytes in vivo or in vitro. Strains Tested: C57BL/6, BALB/c, AKR/J, C3H/He Positive: : C57BL/6, BALB/c, AKR/J, C3H/He Negative: None.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Immunogen: Mouse Ly-2 thymocytes. Donor: (LOU X DA) F1 rat. Fusion Partner: Y3/Ag1.2.3.

Clone: YTS169-4

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry Analysis (see Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend cells to a concentration of 2x10e7 cells/ml Media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test. 4. To each tube add 2. 0 µg of this Ab per 10e6 cells. 5. Vortex to ensure through mixing of antibody and cells. 6. Incubate tubes at 4°C for 30 minutes. (It is recommended that the tubes are protectedfrom light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C in Media B. 8. Resuspend the cell pellet in 50 µl ice cold Media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in phosphate buffered saline. (This stains dead cells by intercalatingDNA). Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 ul of 2 M sodium azide in 100 mls. )B. Phosphate buffered saline (pH 7. 2) + 0. 5% bovine serum albumin + sodium azide (100 ulof 2 M sodium azide in 100 mls. )Results - Tissue DistributionMouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 2. 0 µg/10e6 cellsIsotypic Control: PE Rat IgG2b,kPercentage of cells stained above control: Thymus 66. 2%Spleen 19. 6 %Lymph node 14. 0%

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This antibody is photosensitive and should be protected from light.

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 12525

NCBI Accession: NP_001074579

UniProt: P01731