CD44 (CD44) (AA 3-1) antibody

Details for Product No. ABIN114216
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Antigen
Synonyms AU023126, AW121933, AW146109, HERMES, Ly-24, Pgp-1, CD44A, METAA, RHAMM, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1
Epitope
AA 3-1
(24), (18), (15), (15), (12), (12), (11), (10), (10), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(641), (321), (142), (80), (67), (58), (34), (16), (12), (12), (9), (8), (7), (3), (1)
Host
Mouse
(517), (201), (178), (9), (2), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(144), (82), (69), (29), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application
Cytotoxicity Test (CyTox), Flow Cytometry (FACS)
(564), (283), (216), (155), (150), (127), (121), (74), (54), (46), (13), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.5 mL
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Catalog No. ABIN114216
544.50 $
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Immunogen B6 - Ly-1a spleen DONOR: BALB/c spleen FUSION PARTNER: P3-NS1-1-Ag4(NS1/1)
Clone 5034-44-2
Isotype IgG2a
Specificity This monoclonal antibody recognizes the murine alloantigen Ly 24.2. CD44 (Ly 24B.2, Pgp-1.2) is a 95 kDa glycoprotein previously known as phagocytic glycoprotein -1. It has a wide tissue distribution and is found on bone marrow derived cells, lymphocytes and non - lymphoid tissue such as brain, liver, and kidney. There is variation in Ly 24 expression between mice strains. Generally expression by Ly 24.1 strains is high while in Ly 24.2 it is lower (2). The Ly 24 antigen is expressed by T lymphocytes during primary antigen stimulation. This antigen can be used as a marker to identify activated or memory T cells (3,4).
Purification Ascites
Alternative Name CD44
Background CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Alternate names: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
Gene ID 12505
NCBI Accession NP_001034240
UniProt P15379
Research Area Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle
Application Notes Flow cytometry. (see Protocols)Method for Determining percent of positive cells in a poulation. (see Protocols)Method for Depleting a cells poulation of positive lymphocytes. (see Protocols)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte® M Cell Separation Medium. 2. Wash 2 times. 3. Resuspend cells to 1 x 10e6 cells in approximately 50 µl Media A in a microcentrifugetube (ie. 50 µl of cells resuspended to 2 x 10e7 cells / ml). (THE CONTENTS OF 1 TUBEREPRESENTS 1 TEST). 4. To each tube add 50 µl of 1/500 dilution of this Ab(final dilution 1/1000). 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody CLCC30201 (Goat anti-mouse IgG(H+L)-FITC conjugate)@ 1: 700. 9. Incubate tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in Media B. 11. Resuspend the cell pellet in 50 µl ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg / ml in phosphate buffered saline. (This stains dead cells byintercalating DNA). MEDIA: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% bovine serum albumin + sodium azide (100 µlof 2M sodium azide in 100 mls). RESULTS - TISSUE DISTRIBUTION: PROCEDURE: as aboveANTIBODY CONCENTRATION: 1: 1000MOUSE STRAIN: C57BL/6CELL SOURCE: PERCENT STAININGThymus: 8. 8%Spleen: 36. 1%Lymph Node: 17. 9%
Restrictions For Research Use only
Format Liquid
Reconstitution Reconstitute with 0.5 mL of cold distilled water.
Precaution of Use Use of Sodium Azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Supplier Images
anti-CD44 (CD44) (AA 3-1) antibody Mouse anti CD44 (HCAM) (Ly-24B.2) 5034-44.2
General Sutton, Wijffels, Walker et al.: "Genetic and biochemical characterization of antigens encoded by the Ly-24 (Pgp-1) locus." in: Journal of immunogenetics, Vol. 14, Issue 1, pp. 43-57, 1987 (PubMed).

Butterfield, Fathman, Budd: "A subset of memory CD4+ helper T lymphocytes identified by expression of Pgp-1." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1461-6, 1989 (PubMed).

Lynch, Ceredig: "Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation." in: European journal of immunology, Vol. 19, Issue 2, pp. 223-9, 1989 (PubMed).

Budd, Cerottini, Horvath et al.: "Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp-1 glycoprotein concomitant with antigenic stimulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 10, pp. 3120-9, 1987 (PubMed).

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