CD45 antibody (FITC)

Catalog No. ABIN114220

Product Details anti-CD45 Antibody (FITC)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: CD45RB

Purification: Protein G Chromatography

Immunogen: TH2 cell clones, final boost with TH2 clone D10 Fusion Partner: Ag8.653

Clone: 16A

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry. Immunohistochemistry on acetone-fixed frozen sections and paraffin embedded sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Dilution Used: 1. 0 ug/10e6 cellsIsotypic Control: FITC Rat IgG2a

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45 / LCA (PTPRC Products)

Synonyms: PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody

Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Gene ID: 10090

UniProt: P06800

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling