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Integrin, alpha 4 (Antigen CD49D, alpha 4 Subunit of VLA-4 Receptor) (ITGA4) antibody

Details for Product No. ABIN114223, Supplier: Login to see
Antigen
  • LOC100219853
  • CD49D
  • IA4
Reactivity
Mouse (Murine)
276
219
70
11
8
8
7
6
4
4
4
3
3
3
2
1
1
1
1
Host
Rat
215
194
74
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
86
70
33
9
6
4
4
4
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
384
128
91
73
51
34
32
30
24
18
12
6
4
3
3
3
2
2
2
2
2
2
1
Supplier
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Immunogen Peyers Patch HEV binding lymphoma line (TK1)
Clone R1-2
Isotype IgG2b
Specificity CL030 reacts with a4 integrin, which helps to mediate cell-cell and cell-matrix interactions. Tissue Distribution by Flow Cytometry Analysis (see protocols below): Rat Strain: BALB/c Cell Concentration : 1x10e6 cells per tests Antibody Concentration Used: 1:1000 in 50 μL/10 cells Isotypic Control: Rat IgG2b Cell Source Percentage of cells stained above control (see protocols below): TK1 cells 99.3 % Spleen 89.1 % Thymus 92.1 % Bone Marrow 67.4 % Strain Distribution by Flow Cytometry Analysis (see protocols below): Tissue: Spleen Cell Concentration : 1x10e6 cells per tests Antibody Concentration Used: 1:500 in 50 μL Strains Tested: BALB/c, C57BL/6, C3H/He, CBA/J, AKR Positive: BALB/c, C57BL/6, C3H/He, CBA/J, AKR Negative: none
Purification Ascites
Alternative Name CD49d / ITGA4 (ITGA4 Antibody Abstract)
Background Alpha4 integrin combines with beta1 and beta7integrin to form VLA-4 and LPAM-1 (Peyers patch homing receptor) respectively. VLA-4 is expressed on most peripheral lymphocytes, thymocytes and monocytes. LPAM-1 is found on peripheral lymphocytes, but few thymocytes. Fibronectin and VCAM-1 act as ligands for both VLA-4 and LPAM-1. LPAM-1 also binds the mucosal vascular addressin MAdCAM-1. (1)Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
Gene ID 16401
NCBI Accession NP_034706
UniProt Q00651
Research Area CD Antigens, Surface Receptors of Immune Cells, Cell/Tissue Markers, Extracellular Matrix, Signaling
Pathways
Application Notes Flow cytometry (see protocol/specificity). Immunoprecipitation. Immunohistochemistry. (1,2,3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 50ml of a 1: 500-1000 dilution of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at a 1/500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
Restrictions For Research Use only
Format Liquid
Reconstitution Restore with 0.5 mL of cold distilled water.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer. Centrifuge vial before opening.
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin, alpha 4 (Antigen CD49D, alpha 4 Subunit of VLA-4 Receptor) (ITGA4) antibody (ABIN114223) Cell Source: TK1 Cells
Background publications Berlin, Berg, Briskin et al.: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, 1993 (PubMed).

Holzmann, Weissman: "Peyer's patch-specific lymphocyte homing receptors consist of a VLA-4-like alpha chain associated with either of two integrin beta chains, one of which is novel." in: The EMBO journal, Vol. 8, Issue 6, pp. 1735-41, 1989 (PubMed).

Holzmann, McIntyre, Weissman: "Identification of a murine Peyer's patch--specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4 alpha." in: Cell, Vol. 56, Issue 1, pp. 37-46, 1989 (PubMed).