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Details for Product No. ABIN114229

Selectin L (SELL) antibody

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Antigen
Synonyms CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1, Lnhr, Ly-22, Lyam1, Ly-m22, Lyam-1, LECAM-1, AI528707, L-selectin, A.11
Reactivity
»Alternatives Mouse (Murine)
Host
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunoprecipitation (IP), Flow Cytometry (FACS)
Pubmed 6 references available
Catalog no. ABIN114229
Quantity 0.5 mL
Price
533.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Mouse B cell Lymphoma, 38C-14 Donor: Fischer Rat Spleen Fusion Partner: P3 X 63Ag8.653
Clone MEL-14
Isotype IgG2a
Specificity This monoclonal antibody reacts with a 90 kDa protein which is involved with the homing of lymphocytes to peripheral lymph nodes.
Purification Ascites
Alternative Name CD62L / L-Selectin
Background L-selectin is expressed on most T and B lymphocytes, neutrophils, monocytes, eosinophils.1 Pre-incubation of lymphocytes with this antibody completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro2,3,6 and the migration of lymphocytes to lymph nodes in vivo.2,3 Polymorphonuclear cells preincubated with this antibody do not migrate to the inflammatory foci.
Alternate names: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL
Gene ID 20343
NCBI Accession NP_035476.1
UniProt P18337
Application Notes Flow cytometry.3,4,5Immunohistochemistry.4Immunoprecipitation.3,5,6Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For spleen cell preparations, deplete the red bloodcell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 50 µl of a 1/20,000 - 1/50,000 dilution* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at 1: 500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1/32,000 in 50 µl/10e6 cellsIsotypic Control: Rat IgG2aCell Source: Percentage of cells stained above controlThymus: 64. 1%Spleen: 65. 2%Lymph Node: 75. 1%Results - Strain Distribution:
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 0.5 mL of cold distilled water.
Concentration 10.5 mg/mL (as determined by RID)
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Background publications Siegelman, Cheng, Weissman et al.: "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." in: Cell, Vol. 61, Issue 4, pp. 611-22, 1990 (PubMed).

Jalkanen, Bargatze, de los Toyos et al.: "Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells." in: The Journal of cell biology, Vol. 105, Issue 2, pp. 983-90, 1987 (PubMed).

Lewinsohn, Bargatze, Butcher: "Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4313-21, 1987 (PubMed).

Fink, Gallatin, Reichert et al.: "Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation." in: Nature, Vol. 313, Issue 5999, pp. 233-5, 1985 (PubMed).

Reichert, Gallatin, Butcher et al.: "A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes." in: Cell, Vol. 38, Issue 1, pp. 89-99, 1984 (PubMed).

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, 1983 (PubMed).

Alternatives for antigen "Selectin L (SELL)", type "Antibodies"
Hosts (195), (141), (70), (10), (2), (1)
Reactivities (196), (179), (70), (47), (8), (5), (2), (1), (1)
Applications (359), (83), (63), (48), (45), (32), (30), (26), (25), (17), (10), (8), (7), (6), (3), (2), (1)
Conjugates (69), (55), (29), (20), (8), (8), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Epitopes (7), (5), (3), (3), (3), (3), (3), (2), (1), (1)
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